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| 玉米细胞质雄性不育系胞质类型鉴定及花粉败育研究 | |
| 引用本文： | 孙丽芳,邓杰,王霞,赵伟,杨克军,苗兴芬,高树仁. 玉米细胞质雄性不育系胞质类型鉴定及花粉败育研究[J]. 作物杂志, 2016, 32(3): 27. DOI: 10.16035/j.issn.1001-7283.2016.03.006 |
| 作者姓名： | 孙丽芳 邓杰 王霞 赵伟 杨克军 苗兴芬 高树仁 |
| 作者单位： | 黑龙江八一农垦大学农学院,163319,黑龙江大庆 |
| 基金项目： | 农业部公益性项目(201303007);黑龙江八一农垦大学博士科研启动项目(XYB2013-03);黑龙江省青年科学基金(QC2015029) |
| 摘 要： | 为分析化学诱变产生的玉米细胞质雄性不育材料的不育类型,采用田间育性调查、花粉粒活力检测、结果表明,不育系85218A田间败育彻底,I2-KI染色后花粉粒呈淡黄色,属于典型的圆败,PCR扩增为C型不育系,对C型小斑病菌表现中抗,花粉粒败育关键时期为单核后期。该研究拓宽了我国玉米雄性不育种质资源。 |
| 关 键 词： | 玉米 细胞质 不育系 胞质类型 花粉败育 鉴定 |
| 收稿时间： | 2016-01-22 |
Type Identification of Cytoplasmic Male Sterile Line and Study of Pollen Abortion in Maize |
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| Lifang Sun,Jie Deng,Xia Wang,Wei Zhao,Kejun Yang,Xingfen Miao,Shuren Gao. Type Identification of Cytoplasmic Male Sterile Line and Study of Pollen Abortion in Maize[J]. Crops, 2016, 32(3): 27. DOI: 10.16035/j.issn.1001-7283.2016.03.006 | |
| Authors: | Lifang Sun Jie Deng Xia Wang Wei Zhao Kejun Yang Xingfen Miao Shuren Gao |
| Affiliation: | College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing163319,Heilongjiang,China |
| Abstract: | In this study, the fertility investigation in the field, the testing of pollen viability, PCR amplification of cytoplasmic male sterility with specific primers, identification of maydis resistance and paraffin were used to identify the type of cytoplasmic male sterility after chemical mutagenesis. The results showed that the sterile line 85218A was completely aborted in field. It was a typical yellowish round abortion after I2-KI staining, and was a C-type CMS.It showed mid-tolerance to maydis race C. The critical period of pollen abortion was late mononuclear. This study broadened the male sterility of maize germplasm resources. |
| Keywords: | Maize Cytoplasmic Male sterile line Cytoplasmic type Pollen abortion Identification |
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