总酚含量及多酚氧化酶活性与岩黄连愈伤组织褐化的相关性研究

以未褐化的岩黄连愈伤组织为材料,于培养基中添加不同浓度的抗坏血酸,研究抗坏血酸对岩黄连愈伤组织褐化及总酚含量和多酚氧化酶活性的影响。结果表明：在培养第20天,各处理岩黄连愈伤组织的褐化率均低于对照;各处理的多酚氧化酶活性均高于对照,其中抗坏血酸浓度175mg/L的处理多酚氧化酶活性最高,为14.1400U/(g·min),比对照高72.3%;各处理的总酚含量均低于对照,抗坏血酸浓度为175mg/L的处理总酚含量最低,为0.4633μg/mL,比对照低88.3%。相关性分析结果表明,岩黄连愈伤组织褐变率与总酚含量呈正相关,与多酚氧化酶活性呈负相关;而岩黄连愈伤组织多酚氧化酶活性和总酚含量呈极显著负相关。

Study on Total Phenolic Content and Correlation between PPO Activity and Browning of Corydalis saxicola Bunting Callus

With non-browned callus of Corydalis saxicola as materials, the effects of different concentrations of ascorbic acid on browning of Corydalis saxicola callus, total phenolic content and PPO activity were studied by adding them to culture medium in this experiment. The results showed that the browning rates of Corydalis saxicola bunting callus under different ascorbic acid concentrations were lower than that of the control on the 20th day. While the activities of PPO of all the treatments were higher than that of the control, the activity of PPO with concentration of 175mg/L ascorbic acid was the highest with a value of 14.1400U/(g·min), 72.3% higher than the control. The total phenolic contents of all the treatments were lower than that of the control, and the total phenolic content with concentration of 175mg/L ascorbic acid was the lowest with a value of 0.4633μg/mL, 88.3% lower than the control. The correlation analysis showed that the browning rate of callus was positively correlated with the content of total phenols, and negatively correlated with the activity of PPO. The activity of PPO was much negatively correlated with the total phenol content in the callus.