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豌豆愈伤组织及不定芽诱导影响因素的研究
引用本文:张丽娟,陆建英,王昶,闵庚梅,杨晓明. 豌豆愈伤组织及不定芽诱导影响因素的研究[J]. 作物杂志, 2016, 32(3): 33. DOI: 10.16035/j.issn.1001-7283.2016.03.007
作者姓名:张丽娟  陆建英  王昶  闵庚梅  杨晓明
作者单位:甘肃省农业科学院作物研究所,730070,甘肃兰州
基金项目:现代农业食用豆产业技术体系(CARS-09-G8);国家自然科学基金(31460382)
摘    要:
以豌豆品种陇豌1号为试验材料,对愈伤组织和不定芽诱导过程中种子处理、外植体选择、最适培养基、不定芽诱导及生根等因素进行研究。确定了最适的种子消毒处理为75%乙醇消毒30s,0.1%升汞消毒10min。以下胚轴为外植体诱导愈伤组织,最适激素配比为0.2mg/L 2,4-D和1.0mg/L TDZ,愈伤组织诱导率为91.11%。具有分化能力的愈伤组织持续培养在只添加TDZ的培养基上即可诱导出不定芽,但这些不定芽生根率仅为8.89%,为此分析了影响生根的因素,为后续的研究提供依据。

关 键 词:豌豆  愈伤组织  不定芽  
收稿时间:2016-01-18

The Study on the Influence Factors of Callus and Adventitious Bud Induction in Pea
Lijuan Zhang,Jianying Lu,Chang Wang,Gengmei Min,Xiaoming Yang. The Study on the Influence Factors of Callus and Adventitious Bud Induction in Pea[J]. Crops, 2016, 32(3): 33. DOI: 10.16035/j.issn.1001-7283.2016.03.007
Authors:Lijuan Zhang  Jianying Lu  Chang Wang  Gengmei Min  Xiaoming Yang
Affiliation:Crop Institute,Gansu Academy of Agricultural Sciences,Gansu 730070,Lanzhou,China
Abstract:
The pea variety Longwan No.1 was used as experimental material to study the factors in callus and adventitious bud induction process, such as seed treatment,explants selection, optimal medium, adventitious bud and rooted inducing.The results showed that the optimal disinfection treatments for seed was 75% alcohol solution for 30 seconds, and then 0.1% HgCl2 for 10min. Hypocotyl as the explants, the optimal culture medium of callus was 0.2mg/L 2,4-D and 1.0mg/L TDZ with an induction rate of 91.11%. Only the addition of TDZ could induce adventitious bud,but the rooting rate was only 8.89%. We also analyzed the factors affecting the growth of root,which provided the basis for subsequent research.
Keywords:Pea  Callus  Adventitious shoot  
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