遗传群体构建的分子标记检测体系

为了建立遗传群体构建的分子标记检测体系，利用SSR分子标记技术构建了从DNA提取、引物多态性筛选、亲本和子代植株的PCR扩增、扩增产物的电泳检测等4 步检测体系，实验中仅用1 对位于大麦7H染色体上的引物HvWaxy4 就对7 个F1子代植株进行了鉴定，通过鉴定判断出其中3 个植株为F1子代，4 个单株为自花授粉产生的子代。表明SSR标记技术在验证遗传群体构建过程中F1杂交植株真伪的可行性，该方法适用于自花授粉作物的遗传群体构建过程中F1植株的真伪检测。

The Molecular Marker Checking System for Genetic Population Construction

To establish a molecular marker detection system for genetic population construction, a four-step detection system was constructed using SSR molecular marker technology, including DNA extraction, primer polymorphism screening, PCR amplification of parents and offspring, and electrophoresis detection of amplified products. In the experiment, only one pair of primers HvWaxy4 located on barley 7H chromosome was used to detect 7 F1 progenies, three of them were F1 progenies and four were self-pollinated progenies. The results indicated that SSR marker technology was feasible to verify the F1 hybrid plants in the process of genetic population construction, and the method was suitable to detect the authenticity of F1 plants in the process of self-pollinated crops.