树舌发酵浸膏多糖对肉鸡肠黏膜组织结构及肠道免疫细胞的影响

为研究肉鸡日粮中添加树舌发酵浸膏多糖(Polysaccharides from the fermentation concentrate of Ganoderma applanatum(Pers.ex Gray.),GAP)对肉鸡肠黏膜结构及上皮内淋巴细胞和杯状细胞数量的影响,选用1日龄健康AA肉鸡225只,随机分成5个处理组,每组3次重复,每次重复15只。5个处理组分别为空白对照组(基础日粮)、抗生素阳性对照组(基础日粮+5mg/kg黄霉素)和3个GAP组(基础日粮中分别添加0.05%、0.10%和0.20%GAP),试验期42d。结果表明:1)与对照组相比,日粮中添加0.10%和0.20%的GAP可显著增加肉鸡肠绒毛高度(P0.05),降低隐窝深度(P0.05),提高肠绒毛高度和隐窝深度的比值(V/C)(P0.05);2)与对照组相比,日粮中添加0.10%和0.20%GAP显著升高小肠各段的上皮内淋巴细胞和杯状细胞数量(P0.05);3)小肠各段上皮内淋巴细胞和杯状细胞数量随年龄增长而逐渐增多;4)同一日龄各试验组的上皮内淋巴细胞数量从十二指肠到回肠递减,而杯状细胞则呈递增趋势。以上结果提示:在肉鸡日粮中添加GAP可显著改善肉鸡小肠黏膜结构,维持肠道黏膜的正常屏障结构,促进肉鸡健康生长。其中以0.10%GAP添加量效果最好。

Effects of the polysaccharides from the fermentation extract of Ganoderma applanatum on the mucosal structure and intestinal immunocyte of broilers

Experiment was conducted to study the effects of dietary polysaccharides from the fermentation concentrate of Ganoderma applanatum(Pers.ex Gray.) pat on the mucosal structure and number of intraepithelial lymphocytes and goblet cell in the small intestine of broilers.A total of 225 one-day-old healthy AA broilers were randomly divided into 5 groups with 3 replicates of 15 broilers each.The 5 groups were fed with the basal diet or diets added 5 mg/kg Flavomycin or three concentrations of GAP (0.05%,0.10% and 0.20%) during the 42 days study.The results showed that:1) Dietary supplemented with 0.10% and 0.20% level of GAP significantly increased villus height (P <0.05) and V/C (P <0.05),decreased the depth of crypt (P <0.05);2) Dietary supplemented with 0.10% and 0.20% level of GAP significantly increased the number of intraepithelial lymphocyte and goblet cell in each segment of small intestine (P <0.05);3) the number of lymphocytes and goblet cells increased in each segment of small intestine with increasing of age;4) The number of intraepithelial lymphocytes was gradually reduced from duodenum to ileum in the same age,but the Goblet cell number exhibited an increasing trend.It could conclude that dietary supplementation with GAP could improve the structure of small intestinal mucosa,maintain intestinal mucosal barrier function,and promote the healthy growth of broilers.and the best additive amount in the diet was 0.10%.