| 种植转Bt基因棉花不同年限土壤PLFA指纹特征与差异 |
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| 引用本文： | 李永山,范巧兰,刘凯,陈耕,张冬梅,贾峥嵘,柴永峰,席凯鹏,张建诚.种植转Bt基因棉花不同年限土壤PLFA指纹特征与差异[J].中国农业大学学报,2015,20(3):30-37. |
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| 作者姓名： | 李永山 范巧兰 刘凯 陈耕 张冬梅 贾峥嵘 柴永峰 席凯鹏 张建诚 |
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| 作者单位： | 1. 山西省农业科学院棉花研究所,山西运城,044000
2. 山西大学生物工程学院,太原,030006 |
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| 基金项目： | 国家自然科学基金项目(31372143,30871601); 山西省自然科学基金项目(2014011029-2) |
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| 摘 要： | 为研究连续种植转Bt基因棉花对土壤微生物群落结构的影响,从连续种植3年转基因棉花晋棉26和中棉所41大田,采集土壤样品,测定磷脂脂肪酸(Phospholipid fatty acid,PLFA)。结果表明,不同品种和种植年限对不同生育期土壤微生物影响不同,随着种植年限和棉花残茬进入土壤生态系统,转基因棉花土壤细菌占总量的百分数有降低趋势,革兰氏阳性和阴性细菌的比例低于非转基因品种;转基因棉花土壤真菌的比例逐渐增加。说明转基因棉花连续种植可能改变土壤微生物群落结构。
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| 关 键 词： | 转Bt基因棉花 种植年限 磷脂脂肪酸 土壤微生物 群落结构 |
| 收稿时间： | 2014/10/13 0:00:00 |
Effects of consecutive cultivation of Bt transgenic cotton on soil microbial community structure based on the characterization of phospholipids fatty acids |
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| LI Yong-shan,FAN Qiao-lan,LIU Kai,CHEN Geng,ZHANG Dong-mei,JIA Zheng-rong,CHAI Yong-feng,XI Kai-peng and ZHANG Jian-cheng.Effects of consecutive cultivation of Bt transgenic cotton on soil microbial community structure based on the characterization of phospholipids fatty acids[J].Journal of China Agricultural University,2015,20(3):30-37. |
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| Authors: | LI Yong-shan FAN Qiao-lan LIU Kai CHEN Geng ZHANG Dong-mei JIA Zheng-rong CHAI Yong-feng XI Kai-peng and ZHANG Jian-cheng |
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| Institution: | Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China;Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China;Bioengineering College, Shanxi University, Taiyuan 030006, China;Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China;Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China;Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China;Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China;Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China;Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China |
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| Abstract: | The experiments were conducted to evaluate the effects of 3 years consecutive cultivation of Bt transgenic cotton on soil microbial community in field at two sites based on phospholipid fatty acid (PLFA) profiles.Two transgenic Bt varieties (JM26,CCRI41) and one non-Bt cotton variety (JM7) were used in Yanhu district,Yuncheng site,JM26 and JM7 in Xiaxian county site .The results showed that the total microbial PLFA content were different in different stages,variety and years.The bacteria rate and ratio of Gram-positive and Gram-negative in transgenic cotton were lower than that in non-Bt cotton with consecutive cultivation.The microbial community was characterized by the predominance of fungi in the Bt cotton soil.The soil microbial community had been changed in the Bt cotton soil with consecutive cultivation compared with non-Bt cotton soil. |
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| Keywords: | transgenic Bt cotton consecutive cultivation phospholipid fatty acid (PLFA) soil microbe community structure |
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