紫花苜蓿多叶型与三叶型品种同工酶的比较研究

采用聚丙烯酰胺凝胶电泳技术,对国外2种多叶型苜蓿及国内外5种三叶型苜蓿幼苗的5种同工酶进行了测定,将酶谱数量化,应用SPSS统计软件求出各品种间的遗传一致度及欧氏遗传距离,并进行了聚类分析.结果表明:1)测定的5种同工酶中过氧化物酶(PER)、酯酶(EST)、淀粉酶(AMY)具有多态性,综合此3种酶酶谱可以鉴定供试的7个品种;2)2个多叶型品种具有一共同特征酶带PER-4,国内品种的过氧化物酶活性明显高于国外品种;3)品种间的遗传一致度为0.826～0.952,欧氏遗传距离为0.500～1.118,多叶型品种间遗传一致度为0.904,欧氏遗传距离为0.500;4)等级聚类分析结果为,以欧氏遗传距离0.400为分界,供试品种分为3类,2个多叶型品种为一类,其余的5种三叶型品种分为另两类.

Camparative study on isozymes of multifoliolate and trifoliolate Medicago sativa cultivars

Five isozymes of two multifoliolate Medicago sativa cultivars and five trifoliolate Medicago sativa cultivars were analysed using polylamide gel eletrophoresis technique. Genetic similarity and euclidean coefficient between the cultivars, as well as cluster analysis result were worked out by SPSS (Scientific statistical program of society). The experiment showed: 1)PER, EST and AMY were polymorphic, meanwhile the isozymic chromatograph could identify the cultivars in this study; 2)Two multifoliolate cultivars had specific isozyme loci PER-4, and the level of PER activity of the internal cultivars was higher than the external cultivars; 3) Genetic similarity and euclidean coefficient between the cultivars were 0.826-0.952, 0.500-1.118, between the multifoliolate cultivars were 0.904, 0.500; 4) Multifoliolate cultivares were classified for one type, and others for two other types.