| 利用AFLP技术鉴定凤凰单丛古茶树种质资源 |
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| 引用本文： | 晏嫦妤,李家贤,黄建安,赵超艺,黄华林,唐颢,罗军武.利用AFLP技术鉴定凤凰单丛古茶树种质资源[J].广西农业生物科学,2009(1):89-93. |
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| 作者姓名： | 晏嫦妤 李家贤 黄建安 赵超艺 黄华林 唐颢 罗军武 |
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| 作者单位： | 广东省农业科学院茶叶研究所;湖南农业大学园艺园林学院;湖南省天然产物工程技术研究中心; |
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| 摘 要： | 本文采用AFLP技术对34个凤凰单丛古茶树资源进行了种质鉴定分析。结果显示，筛选出多态性较高的5对引物中，引物组合E41M42、E41M39和E41M33可鉴别出供试的全部资源，鉴别效率为100％；有23份资源具有特异带，占供试材料的67．6％。由此研究表明，AFLP技术可以通过特异带、特异的谱带类型、不同引物提供谱带的组合将凤凰单丛古茶树资源区分开来，这对保护凤凰单丛古茶树资源的育种产权、登录新品种、鉴定和检测其种子与苗木的真实性和纯度具有重要的现实意义。
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| 关 键 词： | AFLP 鉴定 凤凰单丛古茶树 种质资源 |
Identification of Fenghuang-Dancong Tea Plants Germplasm Resources by AFLP Technology |
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| Yan Changyu , Li Jiaxian Huang Jian'an , Zhao Chaoyi Huang Hualin Tang Hao Luo Junwu Tea Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou, Colleage of Horticulture , L,scape,Hu'nan Agricultural University,Changsha, Hu'nan Engineering , Technology Research Center of Natural Products.Identification of Fenghuang-Dancong Tea Plants Germplasm Resources by AFLP Technology[J].Journal of Guangxi Agricultural and Biological Science,2009(1):89-93. |
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| Authors: | Yan Changyu Li Jiaxian Huang Jian'an Zhao Chaoyi Huang Hualin Tang Hao Luo Junwu Tea Research Institute Guangdong Academy of Agricultural Sciences Guangzhou Colleage of Horticulture L scape Hu'nan Agricultural University Changsha Hu'nan Engineering Technology Research Center of Natural Products |
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| Institution: | Yan Changyu 1,2 Li Jiaxian 1 Huang Jian'an 2,3 Zhao Chaoyi 1 Huang Hualin 1 Tang Hao 1 Luo Junwu 2 1 Tea Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,510640,2 Colleage of Horticulture , L,scape,Hu'nan Agricultural University,Changsha,410128,3 Hu'nan Engineering , Technology Research Center of Natural Products |
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| Abstract: | The AFLP technology was used to identify the 34 Fenghuang-Dancong tea plants germplasm resources. The result showed that the primer pairs E41M42, E41M39 and E41M33 can distinguish all the 34 cultivars at one time, the identification efficiency was 100%. The result also showed 23 cultivars had unique AFLP fingerprinting, the percentage was 67.6%. The research showed that AFLP technique is a powerful and efficient tool in identification of the Fenghuang-Dancong tea plants, it can identify the Fenghuang-Dancon... |
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| Keywords: | AFLP Identification Fenghuang-Dancong tea plants Germplasm resources |
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