PRRSV和PCV-2共感染小鼠疾病模型的建立

45只6周龄昆明小鼠,随机分为3组:单次攻毒组(A组)、连续攻毒组(B组)和正常对照组(C组)各15只。A组于试验1 d先后接种PRRSV和PCV-2,B组于1,3,5 d接种PRRSV和PCV-2,C组于1 d接种MEM细胞培养液,各组于试验后7,14,21,28,35 d随机选3只采血后处死,采用体质量测定、抗体ELISA、荧光定量PCR、肉眼和显微病理观察等4项检测指标进行模型鉴定。结果显示,与C组相比,A组和B组小鼠体质量增率减慢;肺脏出现典型的间质性肺炎病变;腹股沟淋巴结肿大,淋巴细胞坏死;脾脏淋巴细胞缺失,被膜散在出血;肝静脉淤血;抗体检测均呈阳性;荧光定量PCR检测证实,C组不存在病毒复制,A、B组小鼠被检组织均存在病毒复制,相对病毒含量在淋巴结中较其他组织差异显著(P0.01)。结果表明,试验已成功建立PRRSV和PCV-2共感染昆明小鼠疾病模型。

Establishment of pathological model of Kunming mice coinfected experimentally with PRRSV and PCV-2

Forty five 6-week-old Kunming mice were divided randomly into three groups,one time inoculated group（group A） and consecutive inoculated group（group B） and control group（group C）（each group,n=15）.The group A were inoculated with PRRSV and PCV-2 in the first day,group B were inoculated with PRRSV and PCV-2 in the 1 st,3th,5th d,group C were inoculated with MEM cell culture medium in the first day.Three mice of three groups were executed after the 7th,14th,21th,28th,35th d.Body weight measurement,antibody detection,fluorescent quantification PCR detection,naked eye and micro-pathology observation were used to detect the treated animals.The results showed that compared with group C,the group A and B displayed slow weight gain in mice,typical lung lesions of interstitial pneumonia,inguinal lymph nodes swelling,lymphocyte necrosis,spleen lymphocytes depletion,scattered subcapsular hemorrhage,the liver venous stasis,antibody positive.Fluorescent quantification PCR testing confirmed the existence of viral replication,except the group C.The relative amount of virus in lymph nodes was more than that of other tissues（P0.01）.So the diseased model could be established successfully in Kunming mice coinfected with PRRSV and PCV-2.