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| GPMV NA-1株致弱F基因真核质粒的构建及免疫试验 | |
| 引用本文： | 李国江,张立春,张秀峰,姜成,刘立明,丁壮.GPMV NA-1株致弱F基因真核质粒的构建及免疫试验[J].中国兽医学报,2010,30(11). |
| 作者姓名： | 李国江 张立春 张秀峰 姜成 刘立明 丁壮 |
| 作者单位： | 1. 吉林农业科技学院,吉林,吉林,132101;吉林大学,畜牧兽医学院,吉林,长春,130062 2. 吉林农业科技学院,吉林,吉林,132101 3. 吉林大学,畜牧兽医学院,吉林,长春,130062 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 将本实验室致弱的GPMV F基因克隆到真核表达载体pCI-neo中,构建pCI-rf质粒,经脂质体法转染BHK细胞后,Western blotting检测pCI-rf能正常表达。采用pCI-rf和本实验室构建的含有GPMV的F基因的真核表达质粒pCI-f分别肌注7~9日龄雏鸡,间接ELISA检测抗体,并采用淋巴细胞增殖试验和特异性CTL杀伤活性试验对试验鸡的细胞免疫进行检测,最后做攻毒保护试验。结果表明,pCI-f和pCI-rf能诱导试验鸡产生细胞免疫和体液免疫应答,与LaSota疫苗比较,免疫效果没有显著差异。PCR扩增检测结果显示,未能从免疫雏鸡各组织的基因组中扩增出F基因片段。 |
| 关 键 词： | 鹅副黏病毒 致弱F基因 核酸疫苗 免疫原性 |
Construction and immunization of eukaryotic plasmid of attenuated fusion gene of GPMV NA-1 strain |
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| LI Guo-jiang,ZHANG Li-chun,ZHANG Xiu-feng,JIANG Cheng,LIU Li-ming,DING Zhuang.Construction and immunization of eukaryotic plasmid of attenuated fusion gene of GPMV NA-1 strain[J].Chinese Journal of Veterinary Science,2010,30(11). | |
| Authors: | LI Guo-jiang ZHANG Li-chun ZHANG Xiu-feng JIANG Cheng LIU Li-ming DING Zhuang |
| Institution: | LI Guo-jiang1,2,ZHANG Li-chun1,ZHANG Xiu-feng1,JIANG Cheng1,LIU Li-ming1,DING Zhuang2(1.Jilin Agriculture Science and Technology College,Jilin,Jilin 132101,China,2.College of Animal Science and Veterinary Medicine,Jilin University,Chuangchun 130062,China) |
| Abstract: | The attenuated GPMV F gene was cloned into the eukaryotic expression vector of pCI-neo and the pCI-rf plasmid was obtained.The pCI-rf plasmid was transfected into BHK cells and the fusion protein was detected through Western blotting successfully.The 7 to 9-day-old chicken was immunized intramuscularly with pCI-rf and pCI-f separately.The antibody level was detected by indirect ELISA and the cell immunity was detected by lymphocyte proliferation assay and specific lysis activity of CTL test.The immunized ch... |
| Keywords: | GPMV attenuated fusion gene DNA vaccine immunogenicity |
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