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| 水稻愈伤组织分化培养过程中保护酶活性及其同工酶的变化 | |
| 引用本文： | 吴 顺,萧浪涛,刘 清,沈革志.水稻愈伤组织分化培养过程中保护酶活性及其同工酶的变化[J].中国农学通报,2007,23(9):24-24. |
| 作者姓名： | 吴 顺 萧浪涛 刘 清 沈革志 |
| 作者单位： | 1. 中南林业科技大学生命科学与技术学院,湖南长沙,410004;湖南农业大学植物激素与生长发育重点实验室,湖南长沙,410128 2. 湖南农业大学植物激素与生长发育重点实验室,湖南长沙,410128 3. 上海市农业科学院作物所,上海,201106 |
| 基金项目： | 湖南省科技计划重大项目＂利用基因工程技术改良超级稻米质的研究＂（K041113-22）. |
| 摘 要： | 以湘早籼33号、01早5202和V20B为供试品种，研究了水稻愈伤组织分化培养过程中保护酶活性及其同工酶酶谱的变化。结果表明：在水稻愈伤组织分化过程中，可溶性蛋白质分别在第6天和第12天出现峰值，而这正是胚性细胞形成和绿苗分化的时期。过氧化物酶活性则在接种后12d出现峰值，分化前期，SOD活性变化趋势与POD活性变化趋势相反，推测这两种酶活性的差异与细胞分化决定有关。此外，POD、SOD同工酶酶谱的变化与水稻愈伤组织分化进程紧密相关 |
| 关 键 词： | 致病性大肠杆菌 致病性大肠杆菌 耐药性 优势血清型 监测 |
| 修稿时间： | 2007-07-06 |
Changes of Protective Enzymes Activity and their Isoenzyme During the Course of Calli Differentiation Culture in Rice |
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| Wu Shun,Xiao Langtao,Liu Qing,Shen Gezhi.Changes of Protective Enzymes Activity and their Isoenzyme During the Course of Calli Differentiation Culture in Rice[J].Chinese Agricultural Science Bulletin,2007,23(9):24-24. | |
| Authors: | Wu Shun Xiao Langtao Liu Qing Shen Gezhi |
| Institution: | Wu Shun, Xiao Langtao, Liu Qing, Shen Gezhi College of Life Science and Technology, Central South University of Forestry and Technology 410004 2 Hunan Provincial Key Laboratory ofPhytohormones, Hunan Agricultural University, Changsha 410128; 3 Crop Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106) |
| Abstract: | In order to investigate the changes of the protective enzymes activity and their isoenzyme during the differentiation of rice callus,the culture was conducted by using the Xiang early indica 33,01 early 5202 and V20B.The results as followed:the soluble protein content had a peak value at the 6th and 12th day,that is,forming stage of embryogenic cells and seedling differentiation.Peak value of POD activity appeared at the 12th day after calli inoculation.Changing trend of SOD activity is opposite to POD activity during the calli differentiation.We supposed that the difference of enzyme activity was relative to the determination of cell differentiation.Moreover,changes of POD and SOD isoenzyme was closely relative to the course of calli differentiation in rice. |
| Keywords: | Rice calli Soluble protein POD SOD Isoenzyme |
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