大豆主要抗原蛋白对埃及胡子鲇肌肉营养成分的影响

在室内单循环控温养殖系统中,给初始体质量为(15.14±0.05)g的健康埃及胡子鲇(Clarias lazera)投喂以鱼粉为动物蛋白源,鱼油、玉米油、糊精为能源,纤维素为填充物配制的3种等氮(粗蛋白质量分数为40%)、等能(15.8 MJ/kg)的半精制饲料(其中β-伴大豆球蛋白的添加量为40 mg/g,大豆球蛋白的添加量为60 mg/g),研究了大豆中主要抗原蛋白对埃及胡子鲇肌肉营养成分的影响。饲喂6周后的调查数据表明:在本试验条件下,β-伴大豆球蛋白和大豆球蛋白使埃及胡子鲇肌肉中粗蛋白含量极显著下降(P<0.01),对肌肉中粗脂肪含量、粗灰分含量影响不显著(P>0.05);使肌肉中氨基酸总量、必需氨基酸总量和鲜味氨基酸总量下降,但与对照组差异不显著(P>0.05)。因此,在埃及胡子鲇幼鱼的配合饲料中,β-伴大豆球蛋白的含量超过40 mg/g、大豆球蛋白的含量超过60 mg/g时,对其肌肉营养成分具有一定的影响。

Effects of Main Soybean Antigens on Nutritional Components of Muscle in Clarias leather

A 6-week feeding trial was conducted to evaluate the effects of main soybean antigens on nutritional components of muscle in Clarias leather with the initial weight of(15.14±0.05) g at controlled temperature in single recirculating system. One control diet and two treatment diets containing purified (3-conglycinin (40 mg/g) or glycinin (60 mg/g) were formulated by fish oil, corn oil, Alsace gum (as three energy sources), fish meal (as animal protein source) and cellulose (as plant packing material) on the base of isonitrogenous (40%) and isoenergetic (15.8 MJ/kg) . The results showed that the crude protein content in muscle of Clarias leather fed with β-conglycinin or glycinin significantly declined( P < 0.01) . There were no significant effects of Glycinin or β-congIycinin on the contents of fat, and ash( P > 0.05 ) . Contents of total amino acids, total essential amino acids and total flavor amino acids decreased in muscles of the fish, but there was no significant difference between the treated groups and the control group( P > 0.05) . Soybean antigens had significant effects on the nutritional components in muscle of Clarias leather, when the content of β-conglycinin or glycinin was over 40 mg/g or 60 mg/g in the juvenile fish diets, respectively.