东方田鼠和小鼠感染日本血吸虫后组织与血清蛋白组分差异比较研究

探讨东方田鼠和小鼠感染日本血吸虫前后不同时点组织与血清中蛋白质电泳谱的变化。分别提取东方田鼠感染日本血吸虫(0、7、12、25 d) 和小鼠感染日本血吸虫(0、7、12、45 d)后的肝脏、脾脏、肺脏蛋白及血清进行聚丙烯酰胺凝胶电泳,对结果比较分析并进行组织病理形态学观察。结果显示,小鼠感染日本血吸虫第7和12天后肝脏150 ku附近蛋白质条带较东方田鼠表达量明显增高;东方田鼠在感染日本血吸虫后40~50 ku附近蛋白质条带较正常东方田鼠表达明显上调;感染第7天后肺脏40 ku附近蛋白质条带较其他时间点变化明显;小鼠感染日本血吸虫第7、12和45天后血清中IgG类蛋白质表达量较东方田鼠显著增高。东方田鼠和小鼠在日本血吸虫感染后不同时点间的组织及血清蛋白组分存在差异,东方田鼠肝脏和血清中150 ku附近蛋白条带较其他动物特异,蛋白质表达丰度高,该蛋白可能与东方田鼠抗日本血吸虫相关。

Comparative Study of Differences of Protein Fractions of Tissue and Serum after Cercariae Infect Microtus fortis and Mice

To observe and compare the differences of protein fractions of tissue and serum at different time points after cercariae infecting Microtus fortis and mice.The liver,spleen,lung and serum were collecting from cercariae infecting Microtus fortis at different time points(0,7,12,45 days post-infection) and mice(0,7,12,45 days post-infection) respectively.The individual soluble proteins were separated by SDS-PAGE and stained with kaomasi blue R-250.The band sizes and their differences were analyzed.Results showed that the 150 ku proteins fractions in liver was more obvious and high expressed after 7,12 days post-infection in mice than in Microtus fortis;the 40 to 50 ku proteins fractions of spleen was high-expressed after cercariae infecting Microtus fortis;the same fractional protein of lung in Microtus fortis showed conspicuous change than other time points;the immunoglobulin in serum of mice was increased significantly after cercariae infecting.There were differences of protein fractions in tissue and serum at different time points after cercariae infecting Microtus fortis and mice.The 150 ku proteins fractions in liver and serum of Microtus fortis was characterized and higher expressed than other animals.It might be an important effective molecule against Schistosoma japonicum infection in Microtus fortis.