猪伪狂犬病病毒北京株的分离鉴定

从北京某猪场疑似猪伪狂犬病发病死亡仔猪脑及内脏中分离到1株病毒并进行了鉴定。该分离毒株接种ST细胞24 h后出现圆缩、聚集、脱落等典型的细胞病变(CPE);分离毒株能够被伪狂犬病病毒标准阳性血清中和;分离病毒接种家兔后,引起家兔出现奇痒等典型的伪狂犬病临床症状;同时根据GenBank公布的PRV的gD基因设计引物并扩增出特异性的目的片段,扩增产物经过测序比较,表明扩增产物序列为猪伪狂犬病病毒基因序列。以上结果证实该病毒为猪伪狂犬病病毒,依据分离地点命名为猪伪狂犬病病毒北京株。

The Isolation and Identification of the Beijing Strain of Pig Pseudorabies Virus

One virus strain was isolated from the samples of brain and internal organs of a death piglet from a hogpen in Beijing. ST cell line shrunk, aggregated and detached from the culture system in 24 h approximately. The isolate could be neutralized by PRV standard positive serum. Rabbits were injected with the isolate and the typical clinical syndrome could be observed, such as extra tickle. According to the gD gene sequences of PRV in GenBank, a pair of primers was designed, a specific fragment was amplified by PCR, the results of sequencing analysis showed that the amplified fragment was the gene sequence of the PRV. All of the evidences indicated that the isolated virus was pseudorabies virus, and was named Beijing strain in accordance with separate location.