一株猪捷申病毒的分离与鉴定

北京地区某猪场猪群疑似感染猪捷申病毒(porcine teschovirus,PTV),为了分离与鉴定该病毒,从采集的脑组织样品中分离出病毒,并从病毒中提取总RNA,根据已报道的引物序列,经RT-PCR扩增得到部分编码主要结构蛋白的VP1基因和高度保守的5'-UTR区基因序列,将这些基因克隆到pEASY-Blunt载体中,经序列测定,与GenBank上的毒株序列进行比对。试验结果表明,分离的毒株与猪捷申病毒同源性为95%~100%。本试验成功分离获得一株猪捷申病毒北京地方流行毒株,命名为 10BJ02株。

Isolation and Identification of Porcine Teschovirus

Some pig groups doubtfullyy infected on porcine teschovirus (PTV) in Beijing area. In order to diagnose this virus's infection, isolated the virus from the brain organization sample, and withdrawed total RNA from the separation virus, according to reported sequence. After using RT-PCR, obtained the part code primary structure protein VP1 gene and highly conservative 5'UTR area gene order, cloned these genes to the pEASY-Blunt vector. The result indicated that the isolated virus and the porcine teschovirus homology is 95% to 100% after compared with GenBank on virus sequence. This experiment successfully isolated a porcine teschovirus by naming as 10BJ02 in Beijing area.