一株鸡源性肠球菌的鉴定及耐药机制分析

本试验旨在鉴定一株从肉鸡体内分离的广谱耐药菌,并从分子生物学角度对该株细菌的耐药机理进行探讨。根据《常见细菌系统鉴定手册》标准对该菌进行培养特性、生化试验鉴定,设计1对16S rDNA通用引物扩增其16S rDNA并测序鉴定;根据该菌的药物敏感性试验结果设计了12对引物扩增其基因组上耐药基因。细菌培养特性和生化试验结果显示该菌为一株肠球菌,PCR扩增出大小为1465 bp的目的片段,药敏试验显示该株细菌耐受多种抗生素,PCR扩增其耐药基因发现存在ant(6)-iv、OXA-10、tetM、CTX-M-1、TEM基因。最终鉴定该株细菌为一株肠球菌,耐药基因的存在是其产生耐药性的原因之一。

Isolation, Identification and Drug Resistance Analysis of a Pathogenic Enterococcus Strain Isolated from Chicken

This experiment was aimed at identifying a Broad-spectrum antibiotic resistant bacteria separated from chickens and discussing the mechanism of antibiotic resistance of the bacteria from the viewpoint of molecular biology. Identify the training characteristics and biochemical experiments of the bacteria based on the standard of Common Bacteria System Identification Manual. A pair of 16S rDNA general primer was designed to amplify 16S rDNA and sequenced. 12 pairs of primer amplify resistance gene was designed based on the results of sensitive method of antibiotic test. The results of training characteristics and biochemical experiments of the bacteria showed that they were Enterococcus. A target set of 1465 bp was amplified by PCR. Sensitivity tests showed that the bacteria tolerate several antibiotics. The results of PCR showed that there exist ant(6)-iv, OXA-10, tetM, CTX-M-1, TEM gene, so it was Enterococcus. The existence of resistance gene was one of the reasons why the bacteria generate resistance characteristics.