3种抗生素处理对灰茶尺蛾内生菌群的影响

为明确不同抗生素对灰茶尺蛾内共生菌群落的影响,分别选用质量浓度2.5 mg·mL^-1的四环素、头孢氨苄、利福平3种抗生素溶液浸泡新鲜茶树叶片持续饲喂灰茶尺蛾幼虫3代,通过16 S rDNA高通量测序技术对内生菌群群落变化展开研究,并进一步基于wsp基因分子标记对灰茶尺蛾沃尔巴克氏体菌去除效果进行检测。结果表明,3种抗生素处理后,灰茶尺蛾菌群落均表现为丰富度增加、多样性减小;四环素和利福平饲喂处理能够成功将灰茶尺蛾沃尔巴克氏体菌去除,进而建立了不携带沃尔巴克氏体菌的灰茶尺蛾种群,为进一步开展沃尔巴克氏体菌的功能研究奠定了基础。

Effect of Three Antibiotic Treatments on Bacterial Endosymbiont Community of Ectropis Grisescens Warren

In order to study the effects of three antibiotics on bacterial endosymbiont community of Ectropis grisescens,relative experiment was carried out with three antibiotics(tetracycline,cefalexin and rifampicin)at the concentration of 2.5 mg·mL-1.Larvae of E.grisescens were fed the tea leaves with antibiotics for continuous 3 generations.Variation of endosymbiont community was studied using 16 S rDNA Illumina MiSeq technique.Besides,the Wolbachia of E.grisescens were detected basing on wsp gene.The results show that abundance and diversity of endosymbiont community in E.grisescens were increased and reduced,respectively under antibiotic treatments.The tetracycline and rifampicin could effectively remove Wolbachia from E.grisescens.Further,the population of E.grisescens without Wolbachia was established,which laid the foundation for the study on function of Wolbachia.