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| 转Bar基因抗除草剂两系杂交早稻恢复系Bar 68-1的培育研究 | |
| 引用本文： | 肖国樱,唐俐,袁定阳,邓晓湘,袁隆平,辛世文.转Bar基因抗除草剂两系杂交早稻恢复系Bar 68-1的培育研究[J].杂交水稻,2007,22(6):57-61. |
| 作者姓名： | 肖国樱 唐俐 袁定阳 邓晓湘 袁隆平 辛世文 |
| 作者单位： | 1. 中国科学院,亚热带农业生态研究所,湖南,长沙,410125;国家杂交水稻工程技术研究中心,湖南,长沙,410125 2. 国家杂交水稻工程技术研究中心,湖南,长沙,410125 3. 香港中文大学,生物系,香港,沙田 |
| 基金项目： | 香港AoE项目;中国科学院知识创新工程项目;湖南省科技攻关项目 |
| 摘 要： | 通过基因枪转化方法将抗除草剂基因Bar转入早籼稻两系恢复系D68中,育成了转基因抗除草剂恢复系Bar 68-1和杂交早稻组合香125S/Bar 68-1;Southern杂交检测和除草剂抗性鉴定结果显示,它们具有抗除草剂特性并能稳定遗传。转基因抗除草剂杂交稻组合香125S/Bar 68-1和对照香两优68(香125S/D68)相比,株高显著降低,直链淀粉含量极显著降低,其它农艺、品质和产量性状没有明显变化。 |
| 关 键 词： | 两系杂交水稻 转Bar基因水稻 抗除草剂恢复系 选育 |
| 文章编号： | 1005-3956（2007）06-0057-05 |
| 收稿时间： | 2007-04-05 |
| 修稿时间： | 2007年4月5日 |
Studies on Development of Bar 68 - 1, a Bar-transgenic Restorer Line with Herbicide Resistance of Two-line Early Hybrid Rice |
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| XIAO Guo-ying,TANG Li,YUAN Ding-yang,DENG Xiao-xiang,YUAN Long-ping,Samuel S. M. Sun.Studies on Development of Bar 68 - 1, a Bar-transgenic Restorer Line with Herbicide Resistance of Two-line Early Hybrid Rice[J].Hybrid Rice,2007,22(6):57-61. | |
| Authors: | XIAO Guo-ying TANG Li YUAN Ding-yang DENG Xiao-xiang YUAN Long-ping Samuel S M Sun |
| Abstract: | A herbicide resistant gene Bar was transferred into a two-line indica restorer line D68 by particle bombardment,and the developed transgenic restorer line Bar 68-1 and its hybrid Xiang 125S/Bar 681 showed herbicide resistance and stable inheritance based on the Southern blotting analysis and the identification of herbicide resistance.When the transgenic hybrid rice Xiang 125S/Bar 68-1 was compared with the check hybrid rice Xiang 125S/D68,the plant height and amylose content of the transgenic hybrid rice were decreased significantly at 5% and 1% levels,respectively,but all of the other major agronomic and yield characters were not statistically different from each other. |
| Keywords: | Bar 68-1 |
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