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紫薄鳅染色体核型分析
引用本文:孟妍,方翠云,祖国掌,唐燕高,徐大宝,洪斌.紫薄鳅染色体核型分析[J].农学学报,2011,1(4):48-51.
作者姓名:孟妍  方翠云  祖国掌  唐燕高  徐大宝  洪斌
作者单位:1. 安徽农业大学生命科学学院,合肥,230036
2. 安徽农业大学动物科技学院,合肥,230036
3. 安徽铜陵县水产开发总公司,安徽铜陵,244000
基金项目:铜陵市2010年度科技计划项目
摘    要:为研究分析长江铜陵地区紫薄鳅染色体的数目及核型,以紫薄鳅头肾为材料,采用植物血凝素(PHA)和秋水仙素体内直接注射培养,空气干燥法制片制备紫薄鳅染色体标本,对紫薄鳅的染色体核型进行分析。核型分析的结果表明:紫薄鳅二倍体染色体数目为2n=50,核型公式为:6m+10sm+8st+26t,臂数为66。研究结果丰富了紫薄鳅细胞遗传学参数,为紫薄鳅杂交育种提供理论依据。

关 键 词:体制创新  体制创新  
收稿时间:2011/4/18 0:00:00
修稿时间:2011/6/1 0:00:00

A Preliminary Study on Chromosome Preparaation and Karytype of Leptobotia taeniops
Meng Yan,Fang Cuiyun,Zu Guozhang,Tang Yangao,Xu Dabao,Hong Bin.A Preliminary Study on Chromosome Preparaation and Karytype of Leptobotia taeniops[J].Journal of Agriculture,2011,1(4):48-51.
Authors:Meng Yan  Fang Cuiyun  Zu Guozhang  Tang Yangao  Xu Dabao  Hong Bin
Institution:1 College of Life Science,Anhui Agricultural University,Hefei 230036,Anhui,China; 2 College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,Anhui,China; 3 Development Corporation of Aquatic Product of Tongling County,Tongling 244000,Anhui,China)
Abstract:To study the chromosome number and karyotype of Leptobotia taeniops,Leptobotia taeniops was involved to make chromosome samples through invivo direct injection culture method of phaseolus vulgaris agglutinin(PHA)and colchicines and air drying method.The total chromosome number of the fish was 50,the karyotype formula was 2n=6m+10sm+8st+26t,and NF=66.No visible evidence was found on po1yploidyc hromosome and sex chromosome.This study provided Leptobotia taeniops with cytogenetics paramete,and offered a theoretical basis for Cross breeding of Leptobotia taeniops.
Keywords:Leptobotia taeniops  Chromosome  Karyotype
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