| 一株拮抗香蕉枯萎病的青霉菌的分离及鉴定 |
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| 引用本文： | 王宇光 卢 娟 孙建波 夏启玉 卢雪华 张 欣. 一株拮抗香蕉枯萎病的青霉菌的分离及鉴定[J]. 中国农学通报, 2011, 27(4): 178-182 |
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| 作者姓名： | 王宇光 卢 娟 孙建波 夏启玉 卢雪华 张 欣 |
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| 作者单位： | 1. 中国热带农业科学院生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101
2. 中国热带农业科学院环境与植物保护研究所,海南,儋州,571737 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项 |
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| 摘 要： | 从海南土壤中分离到一株对香蕉枯萎病病原菌有较强拮抗作用的霉菌菌株QW1，对该菌株进行了显微特征、菌落特征以及26S rDNA序列分析。该菌株在土豆葡萄糖培养基上生长的内生菌丝无横隔膜；孢子梗顶端膨大成帚状；单个分生孢子为椭圆形，蓝色；产有性孢子。菌落生长开始为白色，培养成熟后，气丝逐渐因产生孢子由白色变蓝色。以26S rDNA序列为基础构建了相关种属在内的系统发育树，其与青霉菌株的26S rDNA序列的同源性大于为99%。将该菌株鉴定为青霉菌。将该菌株和香蕉枯萎病病菌在土壤中进行了共培养研究，结果表明，该菌株在土壤中有较强的生长力，并表现出较好的对香蕉枯萎病的抗性。
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| 关 键 词： | 科技 科技 农业产业化 战略 |
| 收稿时间： | 2010-07-06 |
| 修稿时间： | 2010-08-25 |
Isolation and Identification of a Penicillium Strain against Banana Wilt Disease Caused by Fusarium Oxysporum f. sp. Cubense |
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| Wang Yuguang,Lu Juan,Sun Jianbo,Xia Qiyu,Lu Xuehua,Zhang Xin. Isolation and Identification of a Penicillium Strain against Banana Wilt Disease Caused by Fusarium Oxysporum f. sp. Cubense[J]. Chinese Agricultural Science Bulletin, 2011, 27(4): 178-182 |
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| Authors: | Wang Yuguang Lu Juan Sun Jianbo Xia Qiyu Lu Xuehua Zhang Xin |
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| Affiliation: | Wang Yuguang1,Lu Juan1,Sun Jianbo1,Xia Qiyu1,Lu Xuehua1,Zhang Xin2(1Key Laboratory of Tropical Crop Biotechnology,Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,2Environment and plant protection institute,Dan Zhou Hainan 571737) |
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| Abstract: | In order to get some antagonistic microorganism against banana wilt disease caused by fusarium oxysporum f.sp.cubense race 4(FOC4)from soil in Hainan province.Antagonistic microorganism QW1 was isolated by flat confrontation culture from soil in Hainan province.The morphological characteristics,colony characteristics and 26S rDNA sequences analysis of QW1 were studied.Moreover,QW1 and FOC4 wereco-cultured in soil for one month.The results showed that:QW1 produced sexual spores.The substrate myceliums had no... |
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| Keywords: | Penicillium banana wilt disease isolation identification co-culture |
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