| 金丝桃素体外抗H9N2亚型禽流感病毒活性研究 |
| |
| 引用本文： | 韦兰萍,陈建新,何子华,陆春妮,黎金凤,黄显会,方炳虎.金丝桃素体外抗H9N2亚型禽流感病毒活性研究[J].动物医学进展,2010,31(12). |
| |
| 作者姓名： | 韦兰萍 陈建新 何子华 陆春妮 黎金凤 黄显会 方炳虎 |
| |
| 作者单位： | 华南农业大学兽医学院,广东广州,510642 |
| |
| 摘 要： | 研究金丝桃素体外抗H9N2亚型禽流感病毒的活性。用MTT法观察金丝桃素预防、治疗、以及体外直接作用15 min 4种给药方式对感染H9N2亚型禽流感病毒的细胞的保护效果,以及用荧光酶标法测定金丝桃素对禽流感病毒神经氨酸酶的抑制活性。结果表明,金丝桃素的4种给药方式在体外对感染禽流感病毒的细胞的的保护率分别为6.43%、5.31%、39.89%、69.04%。金丝桃素对禽流感病毒神经氨酸酶活性的抑制,IC50为0.58 mg/mL。表明金丝桃素对感染H9N2亚型禽流感病毒的细胞有一定保护的作用,且对神经氨酸酶的活性有较显著的抑制作用。
|
| 关 键 词： | 金丝桃素 流感病毒 H9N2亚型 神经氨酸酶 |
Effect of Hypericin on Influenza Virus H9N2 in Vitro |
| |
| WEI Lan-ping,CHEN Jian-xin,HE Zi-hua,LU Chun-ni,LI Jin-feng,HUANG Xian-hui,FANG Bing-hu.Effect of Hypericin on Influenza Virus H9N2 in Vitro[J].Progress In Veterinary Medicine,2010,31(12). |
| |
| Authors: | WEI Lan-ping CHEN Jian-xin HE Zi-hua LU Chun-ni LI Jin-feng HUANG Xian-hui FANG Bing-hu |
| |
| Institution: | WEI Lan-ping,CHEN Jian-xin,HE Zi-hua,LU Chun-ni,LI Jin-feng,HUANG Xian-hui,FANG Bing-hu(College of Veterinary Medicine,South China Agricultural University,Guangzhou,Guangdong,510642,China) |
| |
| Abstract: | To investigate anti-viral effects of hypericin on infuenza virus H9N2 in vitro,using four different administeation ways,anti-viral effects of hypericin against H9N2 were observed by MTT assay.Florometric assay was used to evaluate the inhibitory effect of hypericin on neuraminidases from influenza virus in vitro.This study showed that hypericin had multiple effects on H9N2 virus,such as prevention effect on virus adsorption,inhibition effect on virus proliferation after adsorption,direct killing virus and t... |
| |
| Keywords: | hypericin Influenza virus H9N2 neuraminidases |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
