百合鳞茎不定芽诱导与增殖配方优化

以百合‘辛普隆’(‘Simplon’)和‘黄色风暴’(Lilium‘Yelloween’)的鳞茎为材料,MS为基本培养基,在(25±2)℃,暗培养下,采用正交试验设计方法研究百合鳞茎不定芽的诱导与增殖。结果表明:①NAA 0.2 mg·L-1有利于‘辛普隆’鳞茎诱导,诱导率80.21%,平均诱导鳞茎2.86个;②NAA 0.4 mg·L-1有利于‘黄色风暴’鳞茎诱导,诱导率46.41%,平均诱导鳞茎2.40个;③蔗糖是影响‘辛普隆’鳞茎增殖主要因子,6-BA是影响‘黄色风暴’鳞茎增殖的主要因子;④MS+6-BA 1.0 mg·L-1+NAA 0.1 mg·L-1+Ad 5 mg·L-1+蔗糖60 g·L-1组合有利于‘辛普隆’鳞茎增殖,增殖率2.22;MS+6-BA 1.5 mg·L-1+NAA 0.1 mg·L-1+Ad 10.0 mg·L-1+蔗糖40 g·L-1有利于‘黄色风暴’鳞茎增殖,增殖率4.5。

Adventitious Bud Induction of Lily Bulb and Proliferation Medium of the Optimization of the Formula

The lily squamas of ＇Simplon＇ and ＇Yelloween＇ were used as materials and MS as basic medium , the adventitious bud induction and proliferation of Lily Bulb were studied using Orthogonal experiment design under the condition of （25 ＋ 2 ） ℃, dark culture. The results showed that （1） NAA 0.2 mg·L^-1 avail adventitious bud induction of ＇Simplon＇ , inductivity 80.21%, the average induced bulb 2.86; （2） NAA 0.4 mg·L^-1 avail adventitious bud induction of ＇Yelloween＇ , inductivity 46.41%, the average induced bulb 2.40; （3） Sucrose is the main factor of the proliferation of ＇Simplon＇, 6-BA is the main factor of the proliferation of ＇Yelloween＇ ; （4） MS ＋ 6-BA 1.0 mg·L^-1 ＋ NAA 0.1 mg·L^-1＋ Ad 5 mg·L^-1 ＋ Sucrose 60 g·L^-1 avail the proliferation of ＇Simplon＇, inductivity 2.22; MS ＋ 6-BA 1.5 mg·L^-1 ＋ NAA 0.1 mg·L^-1 ＋ Ad 10.0 mg·L^-1 ＋ Sucrose 40 g·L^-1 avail the proliferation of ＇Yelloween＇, inductivity 4.5.