Apoplastic redox metabolism: Synergistic phenolic oxidation and a novel oxidative burst |
| |
| Authors: | C Jacyn Baker Daniel P Roberts Norton M Mock Bruce D Whitaker Kenneth L Deahl Andrey A Aver&#x;yanov |
| |
| Institution: | aMolecular Plant Pathology Laboratory, Bldg. 004 Rm. 119, USDA, Beltsville, MA 20705, USA;bSustainable Agricultural Systems Laboratory, USDA, Beltsville, MA 20705, USA;cProduce Quality and Safety Laboratory, USDA, Beltsville, MA 20705, USA;dVegetable Laboratory, USDA, Beltsville, MA 20705, USA;eResearch Institute of Phytopathology, B. Vyazemy, Moscow Region 143050, Russia |
| |
| Abstract: | The plant apoplast is an important mediator of communication between the cell cytoplasm and its surroundings. Plant cell suspensions offer a convenient model system to gain insight into apoplastic physiology. Here, we describe a novel phenomenon that took place when two naturally occurring phenolics were added together to either soybean or tobacco cell suspensions. Acetosyringone (AS) and/or hydroxyacetophenone (HAP), phenolics found in the extracellular/apoplast of tobacco cells, were added to soybean or tobacco cell suspensions undergoing an oxidative burst. Individually, AS appeared to be utilized as a typical peroxidase substrate to scavenge hydrogen peroxide, while HAP was utilized at a much lower rate. However, when added together the rate of utilization of both phenolics increased and surprisingly resulted in the production of hydrogen peroxide. We have further characterized this novel phenomenon in suspension cells. This study demonstrates that certain phenolics in plants can cause co-oxidation which, as in animals, could alter the structure and bioactivity of surrounding phenolics. |
| |
| Keywords: | Co-oxidation Phenolics Glycine max Nicotiana tabacum Pseudomonas syringae Oxidative burst Hydrogen peroxide Apoplast Redox metabolism Suspension cells |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
