暹罗芽孢杆菌ZHX-10的分离鉴定及其对花生白绢病的生防效果

为筛选可用于防治花生白绢病的生防菌株，采用稀释涂布平板法，从土传病害发生严重的花生田中分离 获得了1 株拮抗细菌ZHX-10，结合形态学观察、生理生化鉴定及16S rDNA、gyrB 序列分析对其进行了种类鉴定，并 初步测定了ZHX-10的菌悬液、挥发性气体、发酵液对白绢病菌的抑制效果。结果表明：ZHX-10菌株为暹罗芽孢杆 菌Bacillus siamensis，其菌悬液、挥发性气体和发酵液均能有效抑制白绢病菌菌丝的生长；同时离体叶片接菌实验发 现ZHX-10菌悬液产生的挥发性气体能够有效降低白绢病的发病情况。研究结果表明，ZHX-10 在白绢病菌生物 防治中具有较好的应用潜力和较高的研究价值。

Isolation and identification of Bacillus sinensis ZHX-10 and analysison its biological control activities against Sclerotium rolfsii

In order to screen biocontrol strains for southern stem rot, a bacteria ZHX-10 was isolated from pea? nut field with serious soil-borne diseases by dilution plate method. The strain ZHX-10 was identified based on mor? phological characteristics, physiological, biochemical characteristics, 16S rDNA and gyrB sequence analysis. The inhibitory effects of bacterial suspension, volatile gas, fermentation broth from strain ZHX-10 against Sclerotium rolfsii Sacc. were preliminarily determined. The results showed that ZHX-10 was Bacillus siamensis, and its suspen? sion, volatile gas and fermentation broth could effectively inhibit the growth of Sclerotium rolfsii Sacc.. At the same time, it was found that the volatile gas produced by ZHX-10 suspension could effectively reduce the incidence of Sclerotium rolfsii Sacc. on peanut leaves. It indicated that strain ZHX-10 had high research value and potentials for biological control of Sclerotium rolfsii Sacc.