不同光质LED光源对鱼腥草幼苗膜脂过氧化及抗氧化酶的影响

以鱼腥草为材料,以日光灯处理为对照,分析了不同光质LED光源(白、红、黄、绿、蓝LED灯)对鱼腥草叶片活性氧、类黄酮含量及抗氧化酶的影响。结果表明,蓝光下叶片中TBARS(硫代巴比妥酸)含量最高,较对照高30.18%,与希夫试剂染色结果相一致;过氧化氢产生最多,较对照高190.21%,与DAB染色结果一致;超氧阴离子产生速率在蓝光下最高,为对照组的1.54倍,白光下最低;蓝、绿光下脯氨酸含量是对照的2倍;蓝光和黄光处理下,黄酮含量较对照提高了21.4%。蓝、绿光提高了SOD2同工酶的表达,且在蓝光下最高;蓝、绿、红、黄光处理分别诱导了更多的POD基因位点的表达;不同LED光质均提高了CAT抗氧化酶活性,其中蓝、绿光下CAT同工酶的表达量明显高于对照。综上所述,蓝色光处理可以有效提高幼苗的抗氧化能力,可为鱼腥草品质的控制提供参考。

Effect of LED light source on membrane lipid peroxidation and antioxidant enzyme in Houttuynia cordata Thunb. seedlings

In this study, Houttuynia cordata Thunb. was used as plant material, and the group with fluorescent lamp treatment was the control group. The effects of LED light with different light quality(white, red, yellow, green and blue LED lights)on reactive oxygen species, flavonoids content and antioxidant enzymes of Houttuynia cordata Thunb.leaves were analyzed. The results showed that under blue light, the TBARS content was the highest, 30.18% higher than the control group, which was consistent with Schiff reagent staining results. And hydrogen peroxide produced the most,190.21% higher than the control group, which agreed with DAB staining. The superoxide anion production rate was the highest under blue light which was 1.54-fold compared with the control group, and it was the lowest under white light treatment. Proline content was 2-fold to the control group. The content of flavonoids increased by 21.4% in blue and yellow light treatments compared with the control group. Blue and green light enhanced the expression of SOD2 isoenzyme, and it was the highest under blue LED. Blue, green, red and yellow LED induced more POD gene loci expression, respectively. The various LED monochromatic light improved the CAT activity, within them, CAT activity in blue and green light treatment was obviously improved. In conclusion, blue light treatment can effectively improve the antioxidant capacity of seedlings.