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摩西斗管囊霉对连作花生叶片荧光参数及生理指标的影响
引用本文:崔利,郭峰,唐朝辉,张佳蕾,杨莎,王建国,孟静静,耿耘,李新国,万书波.摩西斗管囊霉对连作花生叶片荧光参数及生理指标的影响[J].中国油料作物学报,2020,42(5):851.
作者姓名:崔利  郭峰  唐朝辉  张佳蕾  杨莎  王建国  孟静静  耿耘  李新国  万书波
作者单位:1. 山东省农作物种质资源中心,山东济南,250100; 2. 山东省农业科学院生物技术研究中心,山东济南,250100; 3. 山东省作物遗传改良与生态生理重点实验室,山东济南,250100; 4. 农业农村部华东地区作物栽培科学观测实验站,山东济南,250100; 5. 山东省农业科学院,山东济南,250100
基金项目:国家自然科学基金青年科学基金(31601261);中国博士后基金(2016M592236);山东省重大科技创新工程(2018YFJH0601);山东省 农业科学院农业科技创新工程(CXGC2018D04);国家花生产业技术体系(CARS-13)
摘    要:连作严重影响花生叶片生长发育,导致花生产量下降和品质降低。为探明摩西斗管囊霉(Funneliformis mosseae)对连作花生叶片生长发育的影响,以大花生品种花育22为试料,采用盆栽试验,研究接种F. mosseae 对连作 花生叶片叶绿素荧光参数、光合作用相关酶活性、抗氧化物酶活性、矿物质元素含量以及叶片干重的影响。结果表 明,与未接种相比,接种F. mosseae 显著增加连作花生叶片叶绿素含量,提高高温强光逆境环境中连作花生叶片原 初反应最大量子效率(Fv/Fm)、实际光化学量子效率(ΦPSП)、光化学淬灭系数(qP)和表观光合电子传递速率(ETR)的 值;同时,接种F. mosseae 的连作花生叶片中核酮糖-1,5-二磷酸核酮糖羧化酶/加氧酶(Rubisco)活性在花针期和饱果 期分别增加了26.52%和32.73%,谷胱甘肽过氧化物酶(GSH-PX)活性、抗坏血酸(AsA)和脯氨酸(Proline)含量也显著 提高;另外,接种F. mosseae 显著促进连作花生叶片对氮、磷、钾和钙的吸收,增加了叶片干物质积累。因此,F. moss? eae 能够促进连作花生叶片生长发育,缓解连作障碍对花生地上部分生长的影响。

关 键 词:丛枝菌根真菌  花生  连作障碍  叶绿素荧光  

Effects of Funneliformis mosseae on fluorescence parameters and physiological index of continuous cropping peanut leaves
CUI Li,GUO Feng,ZHANG Jia-lei,YANG Sha,WANG Jian-guo,MENG Jing-jing,GENG Yun,LI Xin-guo,WAN Shu-bo.Effects of Funneliformis mosseae on fluorescence parameters and physiological index of continuous cropping peanut leaves[J].Chinese Journal of Oil Crop Sciences,2020,42(5):851.
Authors:CUI Li  GUO Feng  ZHANG Jia-lei  YANG Sha  WANG Jian-guo  MENG Jing-jing  GENG Yun  LI Xin-guo  WAN Shu-bo
Abstract:Continuous cropping severely decreases peanut yield and quality. In order to investigate the effect of Funneliformis mosseae on the growth and development of peanut leaves under continuous cropping, the large-seed peanut variety‘Huayu 22’was selected, and the potted method was carried out to study the inoculation with F. mos? seae on chlorophyll fluorescence parameters, photosynthetic-related enzyme activity, antioxidant enzyme actives, mineral element content, and leaves dry weight. The results showed that inoculation of F. mosseae significantly in? creased the chlorophyll content, the maximum quantum efficiency (Fv/Fm), the actual photochemical quantum effi? ciency (ΦPSП), photochemical quenching coefficient (qP) and the apparent electron transfer rate (ETR) under high temperature and strong light irradiation condition compared with uninoculated F. mosseae. Meanwhile, the activity of ribulose-1,5-bisphosphate ribulose carboxylase/oxygenase (Rubisco) in the leaves inoculated with F. mosseae sig? nificantly increased by 26.52% in the flowering period and 32.73% in fruiting period compared with the control. Glutathione peroxidase (GSH-PX) activity, ascorbic acid (AsA) and proline content were also significantly in? creased in the leaves of plants inoculated with F. mosseae. In addition, the content of nitrogen, phosphorus, potassi? um ions and calcium in the leaves of peanut inoculated with F. mosseae was higher than those of control. The leaves dry weight were also significantly increased in peanut plants inoculated with F. mosseae. Therefore, F. mosseae can promote the growth and development of continuous peanut leaves and alleviate the effect of continuous cropping ob? stacles on the growth of peanut.
Keywords:arbuscular mycorrhizal fungi  peanut  continuous cropping obstacles  chlorophyll fluorescence  
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