大豆胞囊线虫病抗性候选基因GmRSCN-6 克隆与表达分析

大豆胞囊线虫病（Heterodera glycines，Soybean cyst nematode，SCN）是世界性大豆病害之一，具有致病力 强、传播范围广、休眠体（胞囊）存活时间长等特点。因此，大豆胞囊线虫病极难防治。筛选并利用抗病基因是加快 大豆抗线育种进程的有效途径之一。本文以东农L-10为材料，克隆GmRSCN-6 基因并分析GmRSCN-6蛋白的性 质，同时对该基因在抗感病品种进行表达分析。结果表明GmRSCN-6 在SCN3号生理小种侵染10 d内逐渐上调表 达。3 d时表达量出现小高峰，推测GmRSCN-6 基因响应SCN3号生理小种入侵信号，10 d时GmRSCN-6 基因的表达 量达到最大，且抗病品种中的表达量远高于感病品种，表明GmRSCN-6 基因参与大豆胞囊线虫病3号生理小种抗性 反应。

Cloning and expression analysis of candidate gene GmRSCN-6 for resistance to soybean cyst nematode

Soybean cyst nematode (SCN) is one of the world's soybean diseases. It has the characteristics of strong pathogenicity, wide spread and long survival time of cyst. Therefore, it is very difficult to control soybean cyst nematode disease. Discovering and utilizing disease resistance genes is one of the effective ways to speed up the breeding process of soybean resistance. In this project, GmRSCN-6 gene was cloned from Dongnongl-10 and the properties of GmRSCN-6 protein were determined, at the same time, the expression of GmRSCN-6 in resistant and susceptible varieties was analyzed.The results showed that the expression of GmRSCN-6 gene was up-regulated in? termittently within 10 days of SCN race 3 infection. The expression of GmRSCN-6 reached a small peak at 3 days. It was stimulated that GmRSCN-6 responsed to the invasion hignal of SCN race 3, and the expression of GmRSCN-6 reached the maximum at 10 days, and then the expression of GmRSCN-6 gene in resistant cultivar was much higher than that in susceptible cultivar. The results showed that GmRSCN-6 gene was involved in the resistance of soybean cyst nematode race 3.