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| 辐照对黄原胶分子量及抗氧化活性的影响 | |
| 引用本文： | 李彦杰,哈益明,王锋,李咏富.辐照对黄原胶分子量及抗氧化活性的影响[J].核农学报,2010,24(6):1208-1213. |
| 作者姓名： | 李彦杰 哈益明 王锋 李咏富 |
| 作者单位： | 1. 中国农业科学院农产品加工研究所/农业部农产品加工与质量控制重点实验室,北京,100193;内蒙古工业大学化工学院,内蒙古,呼和浩特,010051 2. 中国农业科学院农产品加工研究所/农业部农产品加工与质量控制重点实验室,北京,100193 3. 上海理工大学医疗器械与食品学院,上海,200093 |
| 基金项目： | 农业部农业公益性行业科研专项经费项目 |
| 摘 要： | 以0~500kGy 60Co γ射线对固态黄原胶进行辐照，研究辐照对黄原胶分子量及还原力、清除1,1-二苯基-2-苦基肼自由基（DPPH·）、超氧自由基（O2-·）及羟自由基（·OH）能力等抗氧化活性的影响。研究表明，10kGy的辐照可使黄原胶粘均分子量从1.71×106增加到2.23×106，超过10kGy后，黄原胶粘均分子量随辐照剂量的增大逐渐减少，500kGy时降为1.59×104。低于10kGy辐照，黄原胶的还原力、对DPPH·和对O2-·的清除率与剂量呈负相关，与对照相比，还原力降低了3.16%，对DPPH·和O2-·的清除率分别从17.37%和18.18%减少到16.87%和8.51%，增大辐照剂量，黄原胶的还原力和对DPPH·和O2-·的清除率与剂量呈正相关，500kGy时黄原胶的还原力比对照增加164.22%，对DPPH·和O2-·的清除率升高至60.79%和30.06%；对·OH清除能力与辐照剂量呈负相关，清除率从0kGy时的9.29%降低到500kGy时的3.63%。辐照可以引起黄原胶聚合或降解，从而导致其分子量及抗氧化活性发生显著变化。 |
| 关 键 词： | 黄原胶 辐照剂量 分子量 抗氧化活性 |
EFFECT OF IRRADIATION ON MOLECULAR WEIGHT AND ANTIOXIDANT ACTIVITY OF XANTHAN GUM |
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| LI Yan-jie,HA Yi-ming,WANG Feng,LI Yong-fu.EFFECT OF IRRADIATION ON MOLECULAR WEIGHT AND ANTIOXIDANT ACTIVITY OF XANTHAN GUM[J].Acta Agriculturae Nucleatae Sinica,2010,24(6):1208-1213. | |
| Authors: | LI Yan-jie HA Yi-ming WANG Feng LI Yong-fu |
| Institution: | 1.Institute of Agro-Food Science and Technology/Key Laboratory of Agricultural Product Processing and Quality Control, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100193； 2.Chemical and Engineering Institute, Inner Mongolia University of Technology, Huhehaoet,Inner Mongolia 010051; 3. School of Medical Instrument an Food Engineering, University of Shanghai for Science &; Technology, Shanghai 200093) |
| Abstract: | Xanthan gum in dry state was treated at different dosages (0～500kGy) of 60Co γ-rays irradiation to investigate the changes of molecular weight and antioxidant activity of Xanthan gum. The molecular weight of Xanthan gum increased from 1.71×106to 2.23×106 at 10kGy dose of irradiation, then decreased to 1.59×104at 500kGy. Reducing activity and scavenging assays of 1,1-diphenyl-2-picrylhydrazyl radical(DPPH·), superoxide anion radical (O2-·) and hydroxyl radical (·OH) were used to evaluate the antioxidant activity of irradiated Xanthan gum. Below 10kGy, reducing activity and scavenging effect on DPPH· and O2-·were negative correlated with irradiation dose, and compared with control, reducing activity decreased by 3.16%, and the scavenging effects on DPPH· and O2-· decreased from 17.37% and 18.18% to 16.87% and 8.51% at 10kGy, respectively. When irradiation dose was higher than 10kGy, reducing activity and the scavenging effect on DPPH· and O2-·of irradiated samples were postive correlated with irradiation dose, and compared with control, reducing activity increased by 164.22%, and scavenging effect on DPPH· and O2-· increased to 60.79% and 30.06% at 500kGy, respectively. The ·OH scavenging capacity of irradiated samples was negative correlated with irradiation dose, and it decreased from 9.29% at 0kGy to 3.63% at 500kGy. Irradiation can cause polymerization or degradation of Xanthan gum molecule,and lead to significant changes of molecular weight and antioxidant activity of Xanthan gum. |
| Keywords: | xanthan gum γ-irradiation dose molecular weight antioxidant activity |
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