Increased microbial catabolic activity in diesel contaminated soil following addition of earthworms (Dendrobaena veneta) and compost

This study sought to assess the influence of compost and earthworms (Dendrobaena veneta) upon the level of hydrocarbon catabolism in petroleum contaminated forecourt soil (extractable petroleum hydrocarbons (EPH) 10 + 1.8 g kg⁻¹ and total 16 United States Environment Protection Agency (USEPA) polycyclic aromatic hydrocarbons (PAH) 1.62 ± 0.5 g kg⁻¹). The catabolic activity of the indigenous microorganisms within uncombined materials (soil and compost) and within the combined treatments (soil plus compost; either with or without earthworms) was assessed by ¹⁴C-radiorespirometry (¹⁴C-hexadecane, ¹⁴C-toluene and ¹⁴C-phenanthrene). Maximum levels of catabolic activity were observed (at the end of the incubation period; 84 d) for all three compounds in the combined contaminated soil, compost and earthworm mixtures. Significant (p < 0.05) enhancement factors (relative to the soil only control) in catabolic activity in the combined treatments (soil:compost (1:0.5)) of 3.6 times, 1.5 times and 3.5 times were observed for ¹⁴C-hexadecane, ¹⁴C-phenanthrene and ¹⁴C-toluene, respectively; with maximum levels of catabolic activity for these substrates being 68.6 ± 1.7%, 37.9 ± 5.3% and 85.9 ± 1.3%.