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| 血管瘤型禽白血病的实验室诊断 | |
| 引用本文： | 罗明星,周碧君,史开志,杨均,汪德生,安廷贵,杨峰.血管瘤型禽白血病的实验室诊断[J].贵州大学学报(农业与生物科学版),2009,28(1). |
| 作者姓名： | 罗明星 周碧君 史开志 杨均 汪德生 安廷贵 杨峰 |
| 作者单位： | 1. 贵州大学,动物科学院,贵州,贵阳,550025 2. 贵州大学,动物疫病研究所,贵州,贵阳,550025 3. 贵阳市农业局,贵州,贵阳,550000 |
| 基金项目： | 贵阳市农业局科学技术计划 |
| 摘 要： | 采用血清学方法和分子生物学技术对某蛋鸡场感染鸡进行实验室诊断,采用禽白血病A或B亚群抗体检测试剂盒对该鸡场的血清样本进行检测,抗体阳性率达到70%(7/10);针对ALV群特异性抗原P27基因进行PCR扩增,血液、肝脏病料样本中核酸的检出率分别为66.7%(4/6)、75%(3/4);取ALV 囊膜糖蛋白gp85 基因的扩增产物进行酶切分析,扩增的目的片断中存在BglⅡ和 SspⅠ酶切位点,BamHⅠ不能切割PCR 产物.试验结果证实本次疫病是由ALV-A 亚群病毒引起的血管瘤型白血病. |
| 关 键 词： | 禽白血病病毒(ALV) 血管瘤型白血病 血清学 PCR鉴定 酶切分析 |
A Laboratory diagnosis of hemangioma type of avian leukosis |
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| LUO Ming-xing,ZHOU Bi-jun,SHI Kai-zhi,YANG Jun,WANG De-sheng,AN Ting-gui,YANG Feng.A Laboratory diagnosis of hemangioma type of avian leukosis[J].Journal of Mountain Agriculture & Biology,2009,28(1). | |
| Authors: | LUO Ming-xing ZHOU Bi-jun SHI Kai-zhi YANG Jun WANG De-sheng AN Ting-gui YANG Feng |
| Institution: | 1.College of Animal Science;Guizhou University;Guiyang Guizhou 550025;China;2.Insitute of Animal Disease;3.Veterinary Station of Guiyang Agricultural Bureau;Guiyang Guizhou 550000;China |
| Abstract: | Using serological method and molecular biology technique,a laboratory diagnosis was carried out with the affected chickens from a layer farm in the present work.The serum samples were detected using avian leukosis virus antiboby test kit-subgroupA,B(ALV-A or-B,respectively).The positive rate of ALV-A or-B antibody was as high as 70%(7/10).The PCR has been performed against P27 gene of ALV group specific antigen and the detection rates of etiological nucleic acid in blood or liver samples were 66.7%(4/6) or ... |
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