| 白菜雄性不育相关基因BcMF3和BcMF4的A9启动子的基因沉默植物表达载体的构建 |
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| 引用本文： | 刘乐承,曹家树.白菜雄性不育相关基因BcMF3和BcMF4的A9启动子的基因沉默植物表达载体的构建[J].长江大学学报,2007,4(2):48-53. |
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| 作者姓名： | 刘乐承 曹家树 |
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| 作者单位： | 浙江大学蔬菜研究所，浙江大学蔬菜研究所 浙江杭州310029 长江大学园艺园林学院，湖北荆州434025，浙江杭州310029 |
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| 基金项目： | 国家自然科学基金项目(30370975)，浙江省重大科技项目(2005C12019-02) |
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| 摘 要： | 以白菜(Brassica campestris ssp.chinensis,syn.B.rapa ssp.chinensis)雄性不育相关基因BcMF3和BcMF4为沉默对象,利用绒毡层组织特异性的A9启动子,构建了反义RNA、RNA干涉和双价反义RNA植物表达载体。结果表明,经过一系列分子操作,获得了A9启动子和目的基因片段,并利用它们构建了5个基因沉默植物表达载体,可用于基因功能验证。
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| 关 键 词： | 白菜 雄性不育 BcMF3 BcMF4 基因沉默 表达载体 |
| 文章编号： | 1673-1409（2007）02-S048-06 |
| 修稿时间： | 2006-10-09 |
Construction of Gene Silencing Plant Expression Vectors of BcMF3 and BcMF4 Genes Related to Male Sterility in Chinese Cabbage-pak-choi (Brassica campestris ssp.chinensis) |
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| LIU Le-cheng Institute of Vegetable Science,Zhejiang University,Hangzhou,Zhejiang ,China,College of Horticulture and Gardening,Yangtze University,Jingzhou,Hubei ,ChinaCAO Jia-shu.Construction of Gene Silencing Plant Expression Vectors of BcMF3 and BcMF4 Genes Related to Male Sterility in Chinese Cabbage-pak-choi (Brassica campestris ssp.chinensis)[J].Journal of Yangtze University,2007,4(2):48-53. |
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| Authors: | LIU Le-cheng Institute of Vegetable Science Zhejiang University Hangzhou Zhejiang China College of Horticulture and Gardening Yangtze University Jingzhou Hubei ChinaCAO Jia-shu |
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| Institution: | LIU Le-cheng Institute of Vegetable Science,Zhejiang University,Hangzhou,Zhejiang 310029,China,College of Horticulture and Gardening,Yangtze University,Jingzhou,Hubei 434025,ChinaCAO Jia-shu |
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| Abstract: | Targeting at BcMF3 and BcMF4 genes,which related to male sterility of Chinese cabbage-pak-choi (Brassica campestris ssp.chinensis var.communis,syn.B.rapa ssp.chinensis var.communis),gene silencing vectors of antisense RNA,RNAi (RNA interference) and bivalent antisense RNA were constructed using tapetum-specific A9 promoter.The results indicated that,after a serial molecular manipulation,A9 promoter and target gene fragments were obtained.Subsequently 5 gene silencing plant expression vectors were constructed.These vectors can be used in future functional validation of the two genes. |
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| Keywords: | Chinese cabbage-pak-choi male sterility BcMF3 BcMF4 gene silencing expression vector |
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