Establishment of a new canine cell line (CCT) originated from a cutaneous malignant histiocytosis |
| |
Authors: | Sakai Hiroki Nakano Hiroko Yamaguchi Ryouji Yonemaru Kayoko Yanai Tokuma Masegi Toshiaki |
| |
Institution: | Department of Veterinary Pathology, Faculty of Agriculture, Gifu University, Gifu, Japan. |
| |
Abstract: | A new canine cell line, named CCT, was established from the cutaneous malignant histiocytosis in a 4-year-old male Borzoi. CCT proliferated with loose adherence and doubling time was approximately 30 hr. When co-cultured with latex beads, CCT phagocytized beads vigorously. Lysozyme and vimentin were positive by immunostaining, and non-specific esterase and acid phosphatase were positive by cytochemical staining. These features indicated the cells had a histiocytic nature. Furthermore, by subcutaneous injection to nude mice CCT could successfully form tumors with the morphological and immunohistochemical features similar to the original tumor. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|