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| 外源ALA对半夏幼苗耐盐性及其总生物碱含量的影响 | |
| 引用本文： | 梁琴,谢英赞,王朝英,方文,罗涛,何平.外源ALA对半夏幼苗耐盐性及其总生物碱含量的影响[J].广东农业科学,2013,40(15):31-35. |
| 作者姓名： | 梁琴 谢英赞 王朝英 方文 罗涛 何平 |
| 作者单位： | 1. 西南大学生命科学学院/三峡库区生态环境教育部重点实验室/重庆市三峡库区植物生态与资源重点实验室,重庆 400715;毕节学院地理与生命科学学院,贵州毕节 551700 2. 西南大学生命科学学院/三峡库区生态环境教育部重点实验室/重庆市三峡库区植物生态与资源重点实验室,重庆,400715 3. 西南大学生命科学学院/三峡库区生态环境教育部重点实验室/重庆市三峡库区植物生态与资源重点实验室,重庆 400715;重庆市林业科学研究院,重庆 400036 4. 西南大学生命科学学院/三峡库区生态环境教育部重点实验室/重庆市三峡库区植物生态与资源重点实验室,重庆 400715;重庆文理学院林学与生命科学学院,重庆 402168 |
| 基金项目： | 中央高校基本科研业务费专项 |
| 摘 要： | 以药用植物半夏二年生幼苗为材料,在100 mmol/LNaCl模拟盐胁迫条件下,测定了在不同浓度外源5-氨基乙酰丙酸(ALA)处理后,半夏幼苗叶片的可溶性蛋白、超氧阴离子(O2.-)、丙二醛(MDA)的含量,以及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性的变化,并对半夏的总生物碱含量进行了测定。研究结果显示,适宜浓度(50 mg/L)的ALA处理,可以显著提高盐胁迫(100 mmol/LNaCl)下半夏幼苗的耐盐能力及其主要药用成分-总生物碱的含量。 |
| 关 键 词： | 半夏 5-氨基乙酰丙酸(ALA) 盐胁迫 生理特性 总生物碱 |
Effect of exogenous 5-Aminolevulinic Acid (ALA) on the salt tolerance and the total alkaloids content of Pinellia ternate (Thunb.) Breit. biennial seedlings |
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| LIANG Qin , XIE Ying-zan , WANG Chao-ying , FANG Wen , LUO Tao , HE Ping.Effect of exogenous 5-Aminolevulinic Acid (ALA) on the salt tolerance and the total alkaloids content of Pinellia ternate (Thunb.) Breit. biennial seedlings[J].Guangdong Agricultural Sciences,2013,40(15):31-35. | |
| Authors: | LIANG Qin XIE Ying-zan WANG Chao-ying FANG Wen LUO Tao HE Ping |
| Institution: | 1(1.School of Life Sciences,Southwest University/Key Laboratory(Ministry of Education) of Eco-environments of Three Gorges Reservoir Region/Chongqing Key Laboratory of Plant Ecology and Resources Research for Three Gorges Reservoir Region,Chongqing 400715,China;2.School of Geography and Life Sciences,Bijie University,Bijie 551700,China;3.Chongqing Academy Forestry,Chongqing 400036,China;4.Department of Forest and Life Sciences,Chongqing University of Arts and Sciences,Chongqing 402168,China) |
| Abstract: | In order to get the method of improving the salt tolerance of seedlings for Pinellia ternate (Thunb.) Breit. under salt stress,we studied physiological characteristics of P. ternate biennial seedlings. Several physiological indexes of P. ternate biennial seedlings were measured treated by 5-aminolevulinic acid (ALA) under salt stress like the contents of total alkaloids, soluble protein, malondialdehyde (MDA) and the mass fraction of superoxide anion(O2-), the activities of superoxide (SOD) , peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) were also measured. The result showed that under the treatment of 50 mg/L ALA solution, the salt tolerance and total alkaloids content of P. ternate biennial seedlings under NaCl stress(100 mmoloL-1) improved significantly. |
| Keywords: | Pinellia ternate (Thunb ) Breit 5-Aminolevulinic Acid (ALA) NaCl stress physiological characteristics total alkaloids |
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