| 从甘蔗种茎茎汁中提取基因组DNA的方法 |
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| 引用本文： | 李建勇,蒙姣荣,陈保善.从甘蔗种茎茎汁中提取基因组DNA的方法[J].中国农学通报,2010,26(14):86-89. |
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| 作者姓名： | 李建勇 蒙姣荣 陈保善 |
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| 作者单位： | 1. 广西大学农学院,南宁,530004
2. 广西大学生命科学与技术学院,南宁,530004;广西亚热带生物资源保护利用重点实验室,南宁,530004 |
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| 摘 要： | 摘 要： 为探索从甘蔗种茎茎汁中提取基因组DNA的有效方法，分别采用CTAB法、SDS-Ⅰ法和SDS-Ⅱ法进行提取，并以叶片为对照。对所提取的DNA质量进行了检测，结果表明：从茎汁中提取DNA时，SDS-Ⅰ法的产率最高。整体而言，种茎中提取到的DNA产率不如叶片高，但其浓度和纯度足以满足下游分子实验的要求，且其操作过程较叶片简便。
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| 关 键 词： | 科技评价 科技评价 科技评估 农业科技 咨询 |
| 收稿时间： | 2010/3/12 0:00:00 |
| 修稿时间： | 2010/4/16 0:00:00 |
Studies on the Methods of Extraction Genomic DNA from Sugarcane Seed-stem Juice |
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| Li Jianyong,Meng Jiaorong,Chen baoshan.Studies on the Methods of Extraction Genomic DNA from Sugarcane Seed-stem Juice[J].Chinese Agricultural Science Bulletin,2010,26(14):86-89. |
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| Authors: | Li Jianyong Meng Jiaorong Chen baoshan |
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| Institution: | 1 Agricultural College of Guangxi University,Nanning 530004;2 College of Life Science and Technology of Guangxi University,Nanning 530004;3 Guangxi Key Laboratory of Subtropical Bioresource Conversation and Utilization,Nanning 530004) |
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| Abstract: | Genomic DNA usually needs to be extracted from sugarcane seed-stem for quarantine.Leaves are mostly used in previous literature reports on this area.Three different methods were taken to extract genomic DNA from sugarcane seed-stem,and the methods were CTAB,SDS-Ⅰand SDS-Ⅱ.Leaves were taken as the control.The quality of the extracted DNA was detected.The results showed that DNA yield of SDS-Ⅰwas the highest.Overall,although the yield of DNA from seed-stem was lower than leaves,but the concentration and purity of DNA from seed-stem were sufficient to meet the requirement of the downstream molecule experiments,and the operation was more convenient than taking leaves as material. |
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| Keywords: | sugarcane seed-stem stem juice genomic DNA |
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