| 青海石鸡血清淀粉酶同工酶酶谱及其活性研究 |
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| 引用本文： | 王勇,晁生玉.青海石鸡血清淀粉酶同工酶酶谱及其活性研究[J].安徽农业科学,2009,37(31):15257-15258. |
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| 作者姓名： | 王勇 晁生玉 |
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| 作者单位： | 1. 青海大学,青海西宁,810016
2. 青海省海西州动物卫生检疫总站,青海德令哈,817006 |
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| 摘 要： | 目的]为青海石鸡的生化遗传学研究提供基础资料。方法]采用聚丙烯酰胺凝胶垂直平板电泳法对39只青海石鸡血清淀粉酶同工酶酶谱进行研究．并利用比色法对其淀粉酶活性进行测定。结果]青海石鸡血清淀粉酶有Amy—1，Amy一32种同工酶，其中Amy-1有AA、AB和BB3种表型，表型频率分别为17．95％、66．67％、15．38％；AB为优势表型，Amy一1A和amy-1B等位基因频率分别为0．5128和0．4872，基因杂合度为0．4997；青海石鸡血清淀粉酶活性为（566．62±112．20）U／L。结论]青海石鸡Amy一1同工酶呈现多态性。Amy．1AB表型的血清淀粉酶活性显著高于Amy-1AA和Amy一1BB表型。
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| 关 键 词： | 青海石鸡 血清淀粉酶 同工酶 活性 |
Study on Isozyme Pattern and Activity of Serum Amylase in Qinghai Alectoris graeca |
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| WANG Yong et al.Study on Isozyme Pattern and Activity of Serum Amylase in Qinghai Alectoris graeca[J].Journal of Anhui Agricultural Sciences,2009,37(31):15257-15258. |
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| Authors: | WANG Yong |
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| Institution: | WANG Yong et al(Qinghai University,Xining,Qinghai 810016) |
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| Abstract: | Objective] The aim of the study was to provide basic date for biochemical genetic study of Qinghai Alectoris graeca.Method] 39 Qinghai Alectoris graeca were used to study isozyme pattern of serum amylase and activity with vertical polypropylene gel electrophoresis and spectrophotometry,respectively.Result] The study showed that there were two isozymes of serum amylase including Amy-1 and Amy-3 in Qinghai Alectoris graeca.There were three kinds of types that were AA,AB and BB in Amy-1 isozyme,and the phen... |
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| Keywords: | Qinghai Alectoris graeca Serum amylase Isozyme Activity |
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