| 蜜蜂磷酸丙糖异构酶基因(AmTpi)全长cDNA的电子克隆及其验证 |
| |
| 引用本文： | 周君,徐剑,相入丽,陆小平.蜜蜂磷酸丙糖异构酶基因(AmTpi)全长cDNA的电子克隆及其验证[J].福建农林大学学报(自然科学版),2009,38(2). |
| |
| 作者姓名： | 周君 徐剑 相入丽 陆小平 |
| |
| 作者单位： | 1. 苏州大学金螳螂城市建设学院,江苏,苏州,215123;吴江市农林局,江苏,吴江,215200
2. 苏州大学金螳螂城市建设学院,江苏,苏州,215123 |
| |
| 摘 要： | 用电子克隆的方法获得蜜蜂(Apis mellifera)磷酸丙糖异构酶基因(triosephosphate isomerase,Tpi),对该基因序列进行克隆及生物信息学分析.结果表明,该基因全长1768 bp,具有完整的开放阅读框架(ORF),编码247个氨基酸的蛋白;其编码的氨基酸序列与果蝇(Drosophila melanogaster)、家蚕(Bombyxmori)、黄粉虫(Tenebrio molitor)、按蚊(Anopheles gambiae)、烟蚜夜蛾(Heliothis virescens)的磷酸丙糖异构酶的蛋白序列具有较高的保守性.
|
| 关 键 词： | 电子克隆 EST数据库 磷酸丙糖异构酶 蜜蜂 |
Electronic cloning and verification of AmTpi cDNA of Apis mellifera |
| |
| ZHOU Jun,XU Jian,XIANG Ru-li,LU Xiao-ping.Electronic cloning and verification of AmTpi cDNA of Apis mellifera[J].Journal of Fujian Agricultural and Forestry University,2009,38(2). |
| |
| Authors: | ZHOU Jun XU Jian XIANG Ru-li LU Xiao-ping |
| |
| Institution: | 1.School of Goldmantis Urban Construction;Soochow University;Suzhou;Jiangsu 215123;China;2.Agriculture and Forestry Bureau of Wujiang City;Wujiang;Jiangsu 215200;China |
| |
| Abstract: | A triosephosphate isomerase(Tpi) gene of honey bee(Apis mellifera) was cloned by electronic cloning method based on the EST sequences from UniGene of NCBI,its sequence was identified by PCR and predicted by bio-informatics method.The results were as follows: the gene full length was 1768 bp;the gene had a complete open reading frame(ORF),which encoded the protein with 247 amino acids,whose nucleotide sequence had high conservation compared with the protein sequences of triosephosphate isomerase in fruit fly... |
| |
| Keywords: | electronic cloning EST database triosephosphate isomerase Apis mellifera |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
