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| 板栗GA20-氧化酶基因的克隆及序列分析 | |
| 引用本文： | 鲍晓兰,杨凯,冯永庆,秦岭. 板栗GA20-氧化酶基因的克隆及序列分析[J]. 北京农学院学报, 2009, 24(2) |
| 作者姓名： | 鲍晓兰 杨凯 冯永庆 秦岭 |
| 作者单位： | 1. 北京农学院植物科学技术学院,北京,102206 2. 北京农学院农业应用新技术北京市重点实验室,北京,102206 |
| 基金项目： | 国家自然科学基金，北京市自然科学基金 |
| 摘 要： | GA20-氧化酶(GA20-ox)是赤霉素生物合成中的限速酶,它能够催化从GA12到GA9及GA53到GA20过程中的3步氧化反应。笔者以板栗(Castanea mollissimaBlume)为材料,应用基因组步移技术克隆GA20-ox基因,获得长度为2 315 bp DNA序列,其中含有一个GA20-ox基因的全长编码区序列,命名为CmGA20ox1。Cm-GA20ox1长度为1 140 bp,且编码区内存在2个内含子,其推定蛋白CmGA20ox1序列长度为379个氨基酸,氨基酸序列比对分析显示CmGA20ox1与山毛榉坚果的FsGA20ox1(AJ420192)相似性为91.03%,系统发育树分析显示CmGA20ox1与山毛榉坚果FsGA20ox1(AJ420192)遗传距离最近。 |
| 关 键 词： | 板栗 PCR 克隆 GA20-氧化酶 |
Cloning and Sequence Analysis of Gibberellin 20-Oxidase Gene in Chestnut |
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| BAO Xiao-lan,YANG Kai,FENG Yong-qing,QIN Ling. Cloning and Sequence Analysis of Gibberellin 20-Oxidase Gene in Chestnut[J]. Journal of Beijing Agricultural College, 2009, 24(2) | |
| Authors: | BAO Xiao-lan YANG Kai FENG Yong-qing QIN Ling |
| Affiliation: | a.Department of Plant Science and Technology;b.New Technological Laboratory in Agricultural Application in Beijing;Beijing University of Agriculture;Beijing 102206;China |
| Abstract: | Gibberellin 20-oxidase(GA20-ox)is an enzyme that can catalyses the three steps,i.e.oxidization from GA12 to GA9and GA53 to GA20 in the synthesis of active GAs and is a key point in the regulation of GA biosynthesis.Genomic walking was used to isolate GA20-ox gene from chestnut(Castanea mollissima Blume)and DNA sequence of 2315 bp was obtained,including entire coding region of one GA20-ox gene that was designated as CmGA20ox1.The entire coding region of CmGA20ox1 was 1 140 bp,including two introns or a deduc... |
| Keywords: | PCR |
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