熟地黄多糖对环磷酰胺诱导的免疫抑制小鼠的免疫调节作用研究

【目的】研究熟地黄多糖对环磷酰胺诱导的免疫抑制小鼠的免疫调节作用,为熟地黄多糖用于临床治疗免疫低下疾病或开发相关保健食品提供科学依据。【方法】选用36只昆明系小鼠,随机分为6组:空白对照组、免疫抑制模型组、黄芪多糖组及熟地黄多糖低、中、高(分别灌胃熟地黄多糖50、100、200 mg/kg)剂量组(PRRPL、PRRPM、PRRPH),每组6只。除空白对照组外,其余各组小鼠均腹腔注射80 mg/kg环磷酰胺,建立免疫抑制模型。造模后各组小鼠分别灌胃相应药物,1 次/d,连续15 d,测定小鼠体重、腹腔巨噬细胞吞噬能力、免疫器官系数、脾淋巴细胞增殖及细胞周期、血清细胞因子含量,观察脾脏和胸腺组织形态学、肠道派氏节数目等免疫指标。【结果】与空白对照组相比,模型组脾脏指数极显著升高(P＜0.01),脾脏红髓、白髓界限不清,脾淋巴细胞数量减少;胸腺指数降低,皮质、髓质不清,结构破坏;脾淋巴细胞增殖能力、小鼠腹腔巨噬细胞吞噬能力及血清白细胞介素1β(IL-1β)、IL-6、γ-干扰素(INF-γ)水平均显著或极显著降低(P＜0.05;P＜0.01);脾淋巴细胞滞留在G0/G1期。与模型组相比,熟地黄多糖能极显著降低小鼠的脾脏指数(P＜0.01),缓解环磷酰胺造成的脾脏组织损坏,熟地黄多糖各剂量组对脂多糖(LPS)诱导的脾淋巴细胞增殖具有极显著的促进作用(P＜0.01),熟地黄多糖各剂量组能显著或极显著提高免疫抑制小鼠血清IL-1β、IL-6、肿瘤坏死因子α(TNF-α)、INF-γ水平(P＜0.05;P＜0.01),缓解淋巴细胞周期滞留,且高剂量组熟地黄多糖能显著提高小鼠腹腔巨噬细胞吞噬功能(P＜0.05)。造模前后及熟地黄多糖对肠道派氏结数量均无显著影响(P＞0.05)。【结论】熟地黄多糖能提高免疫抑制小鼠的免疫功能,可用于治疗免疫低下类疾病药物和保健品的开发。

Study on Immunomodulatory Effect of Prepared Radix Rehmanniae Polysaccharide on Cyclophosphamide-induced Immunosuppression in Mice

【Objective】 This study was aimed to investigate the immunomodulatory effects of prepared Radix Rehmanniae polysaccharide (PRRP) on cyclophosphamide (CTX)-induced immunesuppression in mice,and provide scientific evidence for the clinical use of PRRP on immune decrease diseases and development of dietary supplement.【Method】 Thirty six Kunming mice were randomly grouped into six groups:Blank control group,model group, Astragalus polysaccharide (APS) group,and three PRRP groups,which oral administer 50,100,200 mg/kg PRRP as PRRPL,PRRPM and PRRPH groups,respectively,six mice each group.Except normal control group,mice in other groups were given 80 mg/kg CTX via peritoneal injection to induce immunosuppression situation.After model was prepared,the mice were given drugs,once a day,for continually 15 days.The body weight,phagocytosis of peritoneal macrophage,immune organ index,tissue damage of thymus and spleen,splenic lymphocyte proliferation and cell cycle were measured,productions of cytokines in serum were determined,and Peyer’s patches (PPs) in intestinal were also counted.【Result】 Compared with blank control group,the index of spleen in model group was extremely significantly improved (P＜0.01),red and white pulp divided unclear,the number of splenic lymphocyte was decreased;The index of thymus was reduced,structure was destroyed with unclear cortex and medulla;The splenic lymphocyte proliferation,phagocytosis of peritoneal macrophage and interleukin-1 β (IL-1β),IL-6 and γ-interferon (INF-γ) in serum were significantly or extremely significantly decreased (P＜0.05; P＜0.01);The splenic lymphocytes were arrested in G0/G1 stage.Compared with model group,the spleen index in mice was extremely significantly decreased by PRRP (P＜0.01),the thymus and spleen structures were destroyed by CTX,the splenic lymphocyte proliferation was extremely significantly induced by advanced and lipopolysaccharide (LPS) (P＜0.01);The levels of IL-1β,IL-6,tumor necrosis factor α (TNF-α) and INF-γ in serum were significantly or extremely significantly improved (P＜0.05; P＜0.01);The splenic lymphocyte arrest was adjusted,and the phagocytosis of peritoneal macrophage in PRRPH group was significantly improved (P＜0.05).There were no significant changes of PRRP and before and after modeling on the number of PPs in intestinal (P＞0.05).【Conclusion】 PRRP could accelerate recovery of CTX-induced immunosuppression in mice,and could be used as drugs or dietary supplement for the treatment of immune decrease diseases.