仔鼠哺乳期补充参苓白术散改善抗生素诱导的肠道稳态失衡

旨在探究参苓白术散调节哺乳期母鼠肠道菌群紊乱导致的仔鼠肠道稳态失衡以及减轻仔鼠断奶应激的机制。选取9只妊娠SD大鼠，随机分成空白组(CON组)、抗生素组(ABX组)、参苓白术散组(SLBZS组)。母鼠分娩后，在ABX组和SLBZS组母鼠饮水中添加抗生素，CON组母鼠正常饮食，第14天开始连续一周给SLBZS组仔鼠灌胃参苓白术散水煎液，CON组与ABX组的仔鼠灌胃等量生理盐水。分娩后第14、21天采集仔鼠的粪便进行微生物16S rRNA基因测序，采用Western blot法检测结肠组织中紧密连接蛋白ZO-1和Occludin的表达量，采用苏木精-伊红(HE)染色法对仔鼠结肠组织进行组织病理学观察。结果显示，母鼠抗生素处理导致仔鼠肠道菌群的多样性显著低于CON组(P<0.05),而参苓白术散处理有效缓解仔鼠菌群紊乱，降低了Enterococcus、Sutterella、Shigella等病原菌的相对丰度，并升高了Lactobacillus、Blautia及Akkermansia等菌属的相对丰度；ABX组仔鼠结肠中紧密连接蛋白ZO-1和Occludin的表达量显著低于CON组(P&l...

Supplement of Shenling Baizhu Powder to Offspring Rats during Sucking Improved Intestinal Dyshomeostasis Induced by Antibiotics

This study aimed to investigate the mechanism of Shenling Baizhu powder on improving intestinal homeostasis and relieving weaning stress of newborn rats. Nine pregnant SD rats were randomly divided into three groups, including blank group (CON), antibiotics group (ABX) and Shenling Baizhu powder group (SLBZS). After delivery, antibiotics were added to the drinking water of the female rats in ABX group and SLBZS group, the female rats in CON group were treated normally, and the offsprings in SLBZS group were gavaged with Shenling Baizhu powder decoction for 7 consecutive days from the 14th day after delivery, the offsprings in CON group and ABX group were gavaged with the same amount of normal saline. On day 14 and day 21 after delivery, the fecal samples of the offsprings in each group were collected and analyzed by 16S rRNA gene sequencing. Western blot was used to detect the expression of ZO-1 and Occludin proteins in the colon of the offsprings. Moreover, the histopathological changes of the colon section were detected by hematoxylin and eosin (HE) staining. The results showed that the intestinal microbial diversity in ABX group was significantly lower than that in CON group (P<0.05), and the Shenling Baizhu powder treatment reduced the relative abundance of pathogenic bacteria, such as Enterococcus, Sutterella, and Shigella, while increased the relative abundance of Lactobacillus, Blautia and Akkermansia. The expression of tight junction proteins ZO-1 and Occludin in colon of offspring rats were significantly lower than CON group (P<0.05). After treated with Shenling Baizhu powder, the gut barrier of offspring rats had been improved. The histopathological section of the colon showed obvious inflammatory cell infiltration in the ABX group, while the inflammatory cell infiltration was reduced in the SLBZS group. In conclusion, the use of antibiotics in maternal rats during the period of lactation could change the gut microbes structure in offspring rats, which may affect the permeability of the intestinal mucosa, and has the potential to promote intestinal inflammation. The use of Shenling Baizhu powder on sucking rats could modulate the structure of gut microbiota and increase the expression level of intestinal tight junction protein ZO-1 and Occludin, thus improving the health of intestinal.