| 青蒿组织中快速有效DNA提取方法的研究(英文) |
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| 作者单位： | 湖北民族学院生物科学与技术学院，湖北民族学院生物科学与技术学院 湖北恩施445000 湖北省生物资源保护与利用重点实验室 湖北大学中药生物技术省重点实验室，湖北武汉430062，湖北恩施445000 湖北省生物资源保护与利用重点实验室 湖北大学中药生物技术省重点实验室，湖北武汉430062 |
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| 摘 要： | [Objective] The objective of this study is to explore a rapid and efficient method of extracting genomic DNA from Artemisia abrotanum. [Method] Three methods of Cutting [Method],Liquid Nitrogen [Method] and Quartz Sand [Method] based on SDS method were employed to extract Artemisia abrotanum genomic DNA from tender leaf at seedling stage,tender spike and old leaf at heading stage. The obtained DNAs were detected by absorbance detection,agarose gel and PCR amplification. [Result] Cutting [Method] performed better than the other two methods compared in purity,extracting cycle and cost,accordingly more suitable for PCR amplification. The results also show that young spike is the best material for extracting genomic DNA from Artemisia Annua.
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An Improved Method of Extracting Artemisia abrotanum Genomic DNA |
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| Abstract: | [Objective] The objective of this study is to explore a rapid and efficient method of extracting genomic DNA from Artemisia abrotanum. [Method] Three methods of Cutting Method,Liquid Nitrogen Method and Quartz Sand Method based on SDS method were employed to extract Artemisia abrotanum genomic DNA from tender leaf at seedling stage,tender spike and old leaf at heading stage. The obtained DNAs were detected by absorbance detection,agarose gel and PCR amplification. [Result] Cutting Method performed better than the other two methods compared in purity,extracting cycle and cost,accordingly more suitable for PCR amplification. The results also show that young spike is the best material for extracting genomic DNA from Artemisia Annua. |
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| Keywords: | Artemisia abrotanum DNA extraction Cutting method |
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