| 亚麻脱胶用复合酶高产菌株的诱变选育 |
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| 引用本文： | 田英华,刘晓兰,郑喜群.亚麻脱胶用复合酶高产菌株的诱变选育[J].西北农业学报,2010,19(1):191-193. |
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| 作者姓名： | 田英华 刘晓兰 郑喜群 |
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| 作者单位： | 齐齐哈尔大学,生命科学与工程学院,齐齐哈尔大学,农产品加工黑龙江省普通高校重点实验室,黑龙江,齐齐哈尔,161006 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目 |
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| 摘 要： | 以黑曲霉(Aspergillus niger)HYA4为出发菌株,采用紫外线(UV)进行诱变处理.在最佳紫外线照射剂量120 s的处理条件下,筛选获得了一株亚麻脱胶酶高产突变株黑曲霉A15.经过连续传接5代,该菌株产酶稳定性良好.固体发酵后聚半乳糖醛酸酶活力达到2 854.27 U/g,木聚糖酶活力可达97.01 U/g,分别比出发菌株黑曲霉HYA4提高了59%和26%.
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| 关 键 词： | 诱变 紫外线 黑曲霉 聚半乳糖醛酸酶 木聚糖酶 |
| 收稿时间： | 2009/4/30 0:00:00 |
| 修稿时间： | 2009/5/27 0:00:00 |
Screening of High-yielding Flax Retting Enzyme Mutant |
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| TIAN Yinghu,LIU Xiaolan and ZHENG Xiqun.Screening of High-yielding Flax Retting Enzyme Mutant[J].Acta Agriculturae Boreali-occidentalis Sinica,2010,19(1):191-193. |
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| Authors: | TIAN Yinghu LIU Xiaolan and ZHENG Xiqun |
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| Institution: | TIAN Yinghua,LIU Xiaolan , ZHENG Xiqun(College of Life Science , Engineering,Key Laboratory of Processing Agricultural Products of Heilongjiang Province,Qiqihar University,Qiqihar Heilongjiang 161006,China) |
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| Abstract: | The Aspergillus niger HYA4 was treated by UV,and 120 seconds was the optimum dosage.The high-yielding strain A15 was obtained by screen.The activity of polygalacturonase and xylanase for the fifth subsequent generation of subcultures reached 2 854.27 U/g and 97.01 U/g respectively,which increased 59% and 26% than HYA4. |
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| Keywords: | Mutate UV Aspergillus niger Polygalacturonase Xylanase |
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