| 枣叶片DNA的提取以及AFLP反应体系的建立 |
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| 引用本文： | 文亚峰,何钢.枣叶片DNA的提取以及AFLP反应体系的建立[J].中南林业科技大学学报,2007,27(3):25-28. |
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| 作者姓名： | 文亚峰 何钢 |
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| 作者单位： | 中南林业科技大学资源与环境学院 湖南长沙410004(文亚峰)，中南林业科技大学生命科学与技术学院 湖南长沙410004(何钢) |
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| 基金项目： | 湖南省教育厅优良青年项目;湖南省自然科学基金 |
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| 摘 要： | 扩增片断长度多态性(AFLP)实验过程中,DNA提取质量和适合的PCR扩增反应体系是2个关键因素.利用CTAB法对枣树叶片DNA的提取方法进行探讨.结果表明:改良CTAB法抽提到了较高质量的枣基因组DNA,可用于AFLP实验分析;通过对酶切-连接体系和PCR扩增体系中的关键因素实验分析,建立了适合枣AFLP荧光反应体系,并得到清晰的DNA指纹图谱.
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| 关 键 词： | 经济林栽培 枣 DNA提取 PCR反应体系 |
| 文章编号： | 1673-923X（2007）03-0025-04 |
| 收稿时间： | 2007-02-16 |
| 修稿时间： | 2007-02-162007-05-09 |
DNA Extraction Method and AFLP Reaction System of Ziziphus jujuba |
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| WEN Ya-feng,HE Gang.DNA Extraction Method and AFLP Reaction System of Ziziphus jujuba[J].Journal of Central South Forestry University,2007,27(3):25-28. |
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| Authors: | WEN Ya-feng HE Gang |
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| Institution: | 1. School of Resources and Environment; 2. School of Life Science and Technology, Central South University of Forestry and Technology Changsha 410004, Hunan, China |
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| Abstract: | The quality of genomic DNA and PCR amplification reaction system are the key factors in AFLP experiment.Using CTAB,this paper discusses and finds a novel method for extracting genomic DNA from the leaves of Ziziphus jujuba.This improved CTAB method can receive high quality genomic DNA and is suitable for further AFLP analysis.Some key factors affecting restriction-ligation reaction,preamplification and selective amplification processes were studied and thus a suitable PCR reaction system for Ziziphus jujuba was established.With this system,clear polymorphic fingerprints were obtained. |
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| Keywords: | AFLP |
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