香菇939菌株单核菌丝和双核菌丝多糖产量比较

香菇939菌株的担孢子萌发成的单核菌丝菌落, 根据其特征可分为4种类型: S型(菌丝贴生型) ; A型(粉状致密型) ; R型(菌丝稀薄型) 和H型(绒毛气生型) 。摇瓶液体培养单核菌丝和双核菌丝, 根据其产多糖能力, 可将4种类型单核菌丝分为两组: 第1组S型和R型, 多糖产量和双核菌丝相似; 第2组A型和H型, 多糖产量显著低于第1组。菌丝贴生型单核菌丝菌落S1高产多糖所需的营养和环境条件与其亲本双核菌丝基本相同, 但是, 易利用的碳源葡萄糖、蔗糖和麦芽糖对单核菌丝产多糖有较大的抑制作用, 而对双核菌丝产多糖的影响则较小。单核菌丝对高溶氧水平没有双核菌丝敏感, 高溶氧水平对双核菌丝产多糖有明显的抑制作用。发酵液初始pH值偏酸性对双核菌丝产多糖不利, 而对单核菌丝产多糖影响较小。

Comparing the Lentinan Yield of the Monokaryontic Mycelia to the Dikaryotic Mycelia from Lentinula edodes 939

Lentinan(LN) is a very important immunomodulator. The characteristics of producing LN of monokaryontic mycelia developed from the basidiospores have not been reported. Our research demonstrated that the monokaryontic mycelia colonies developed from basidiospores of Lentinula edodes 939 could be classified into four types: type S, sticking; type A, appressed;type R, rare and thin;type H, fine hair. The four types of the monokaryontic mycelia could divide into two groups based on the LN yield by shaking flack cultivation. The first group included type S and R where LN production was similar to that of the dikaryotic mycelia; the second group included type A and H where LN production was significantly lower than that of the first group. The nutrition and environment required for producing LN of monokaryontic mycelia S1 of type S were basically similar to that of its parent dikaryotic mycelia. However same as glucose, the easily utilized C sources, such as sucrose and maltose, displayed strong repression on S1 for producing LN but weak repression on dikaryotic mycelia. Monokaryontic mycelia were not as sensitive to high dissolved oxygen as dikaryotic mycelia which could be considerably repressed. An initial lower pH of liquid media did not profit dikaryotic mycelia to produce LN, but had little effect on monokaryontic mycelia.