| 外源DNA导入冬瓜条件的优化及其运用 |
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| 引用本文： | 谢大森,何晓明,彭庆务,李华平.外源DNA导入冬瓜条件的优化及其运用[J].分子植物育种,2009,7(3). |
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| 作者姓名： | 谢大森 何晓明 彭庆务 李华平 |
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| 作者单位： | 1. 广东省农科院蔬菜所,广州,510640
2. 华南农业大学资环学院,广州,510640 |
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| 基金项目： | 农业部行业科技计划，广东省科技攻关项目，广州市农业科技项目，现代农业产业技术体系建设专项基金 |
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| 摘 要： | 针对冬瓜(Benincasa hispida(Thunb.)Cogn.)缺乏抗性种质资源的现状,本文开展了冬瓜抗枯萎病种质资源创新研究.采用花粉管通道法将高抗枯萎病的黑籽南瓜DNA导入优质冬瓜材料,以外源DNA剂量、pH值、注入时间、注入方法以及冬瓜品种为因素,通过正交设计,设计处理组合,筛选和优化外源DNA导入冬瓜条件.结果表明:以子房注射法、授粉后24 h、导入浓度为1.2 mg/mL DNA溶液(pn 7.5)的处理,对冬瓜坐果率的影响最小.利用此方法将高抗冬瓜枯萎病的黑籽南瓜总DNA导入冬瓜,经病圃田间筛选和6代自交纯化已获得2份稳定的冬瓜新材料.经苗期人工接种和病圃田间自然抗病鉴定,其抗病性明显提高.所获抗病材料的品质与受体无明显差异.其中1份抗枯萎病材料已运用于育种实践,培育出了抗病、优质粉皮冬瓜"广利1号",目前该品种每年推广面积700 hm2以上.
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| 关 键 词： | 冬瓜 外源DNA 花粉管通道法 正交设计 田间性状 |
Optimization of Parameters for Introduction of Exogenous DNA into Winter Gourd and its Utilization |
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| Xie Dasen,He Xiaoming,Peng Qingwu,Li huaping.Optimization of Parameters for Introduction of Exogenous DNA into Winter Gourd and its Utilization[J].Molecular Plant Breeding,2009,7(3). |
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| Authors: | Xie Dasen He Xiaoming Peng Qingwu Li huaping |
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| Institution: | Xie Dasen 1 He Xiaoming 1 Peng Qingwu 1 Li huaping 2 1 Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,510640,2 College of Natural Resources , Environment,South China Agricultural University |
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| Abstract: | Because there is no germplasm with resistance to Fusarium wilt in winter gourd, the DNA of Cucurbita ficifolia highly resistant to this disease was introduced into a winter gourd cultivar with good agronomic characters through pollen-tube pathway for the improvement of winter gourd germplasm. The effects of the dose/concentration of DNA, pH value, injection timing, and injection methods on the ratio of fruit setting were studied using the orthogonal design. The results showed that the ratio of fruit setting... |
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