不同处理对柿属植物DNA提取产率和品质的影响

基于CTAB法对鄂柿一号、前川次郎、君迁子3份材料进行DNA提取纯化,探讨不同采样时期、不同的纯化及沉淀方法对柿属植物DNA产率和品质的影响.结果表明,不同时期采样对DNA产率影响很大,随叶片生长,DNA产率不断下降,成熟叶片DNA产率仅为幼嫩叶片的16.7％～19.4％；在苯酚氯仿法、高盐乙醚法、CTAB沉淀法中以高盐乙醚法效果最好,可酶切,能有效去除多糖污染,产率较高；4种沉淀方法中,高浓度的盐溶液有助于去除多糖、多酚等杂质,相比单纯的乙醇或异丙醇沉淀可获得更高品质的DNA.另外,不同材料间提取DNA难度存在较大差异.

Effect of Different Treatments on Genomic DNA Extraction in Diospyros spp

The efficiency of many nucleic acid isolation techniques is affected by the presence of plant metabolites such as polysaccharides,polyphones,etc.Based on CTAB DNA extraction method,some treatments with different sampling,purifying and precipitating were studied to preserve the effect on genomic DNA extraction in three materials of Diospyros spp.The results indicated that as the tissue developed,the yield of DNA decreased.Compared with tender shoots,the amount of DNA from mature leaves was 16.7%-18.4%.Among three purification methods,the high salt/water-saturated ether obtained high-quality easily digested DNA with higher yield.In addition,add high concentration salt before organic precipitation is helpful to remove the impurity,could gain higher-quality DNA.The efficiency of DNA extraction form cultivars differs much.