金顶侧耳多糖体外抗肿瘤作用的研究

通过深层发酵获得金顶侧耳菌丝体。用水提法分别提取金顶侧耳菌丝体多糖、胞外(过滤液)多糖和全液(菌丝体 发酵液)多糖。用MTT比色法测定金顶侧耳多糖体外对小鼠S180癌细胞及人结肠低分化腺癌细胞的抑制率。结果表明,金顶侧耳胞外多糖对体外培养的S-180癌细胞有抑制作用,全液多糖和菌丝体多糖无抑制作用。这3种多糖体外对人结肠低分化腺癌细胞有抑制作用,其中金顶侧耳胞外多糖抑制率最高,全液多糖次之,菌丝体多糖最低。

A Study on the Inhibition Effect of Polysaccharides Extracted from Pleurotus citrinopileatus on Tumour Cells in vitro

The mycelia of Plearotus citrmopileatus were cultured through submerged fermentation, and po-lysaccharides were extracted from the mycelia, the filtrate and the fermented broth resp. . While the inhibition rate of P. citrinopileatus polysaccharide for mouse S-180 tumour cells and low-differentiated adenocarcinoma cells of human colon were determined by MTT colorimetry. The experiment results showed that the excellular polysaccharide extracted from the filtrate was proved to have inhibition effect to cultured S-180 cells in vitro, the polysaccharide extracted from the mycelia and the fermented broth have no inhibition effect to cultured S-180 cells in vitro. And it was also proved in our experiment that three kinds of P. citrinopileatus polysaccharide all had antitumour activity to cultured low-differentiated adenocarcinoma cells of human colon in vitro , and that the inhibition rate of the polysaccharide extracted from the filtrate was the highest, followed by the polysaccharide extracted from the fermented broth and the mycelia of P. crtrinopileatus.