Preparation and characterization of blended <Emphasis Type="Italic">Bombyx mori</Emphasis> silk fibroin scaffolds |
| |
Authors: | Waree Tiyaboonchai Pratthana Chomchalao Sutatip Pongcharoen Manote Sutheerawattananonda Prasert Sobhon |
| |
Institution: | (1) Research Center of Material Science, Beijing Institute of Technology, Beijing, 100081, China; |
| |
Abstract: | The aim of this study was to compare physical, mechanical and biological properties of 3-dimensional scaffolds prepared from
Bombyx mori silk fibroin (SF), fibroin blended with collagen (SF/C), and fibroin blended with gelatin (SF/G) using a freeze-drying technique.
The prepared scaffolds were sponge-like structure that exhibited homogeneous porosity with highly interconnected pores. Average
pore size of these scaffolds ranged from 65–147 μm. All biodegradable scaffolds were capable of water absorption of 90 %.
The degradation behavior of these scaffolds could be controlled by varying the amount of blended polymer. The SF/C and SF/G
scaffolds showed higher compressive modulus than that of SF scaffolds which could be attributed to the thicker pore wall observed
in the blended constructs. The less crystalline SF structure was observed in SF/G scaffolds as compared to SF/C scaffolds.
Thus, the highest compressive modulus was observed on SF/C matrix. To investigate the feasibility of the scaffolds for cartilage
tissue engineering application, rat articular chondrocytes were seeded onto the scaffolds. The MTT assay demonstrated that
blending collagen or gelatin into SF sponge facilitated cell attachment and proliferation better than SF scaffolds. The blended
SF scaffolds possessed superior physical, mechanical and biological properties in comparison to SF scaffolds and showed high
potential for application in cartilage tissue engineering. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|