| 柑橘衰退病毒柚分离株的p25/Hinf1 RFLP分析 |
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| 引用本文： | 宋震,徐小峰,杨方云,周常勇.柑橘衰退病毒柚分离株的p25/Hinf1 RFLP分析[J].植物保护,2005,31(4):28-31. |
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| 作者姓名： | 宋震 徐小峰 杨方云 周常勇 |
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| 作者单位： | 中国农业科学研究院柑橘研究所,重庆,400712 |
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| 基金项目： | 人事部留学回国人员科技活动择优资助项目 |
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| 摘 要： | 对123个柑橘衰退病毒柚分离株进行p25/Hinf1 RFLP分析明确:样品中,单一p25/Hinf1 RFLP组群样品占样品总量的82.9%,说明我国田间受侵染柚类中柑橘衰退病毒株系相对较为单一;RFLP组群6的样品占样品总量的79.7%,说明目前柚类生产上的优势流行株属于p25/Hinf1 RFLP组群6,初步认定为强毒株系;柑橘衰退病毒株柚分离株S102、S104、S106、S108属于p25/Hinf1 RFLP组群5,初步认定是弱毒株系。
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| 关 键 词： | 植物病毒学 柑橘衰退病毒 分离株 限制性片段长度多态性分析 |
| 收稿时间： | 2005-01-11 |
| 修稿时间： | 2005-01-11 |
A p25/Hinf1 RFLP analysis of the Citrus tristeza virus isolates infecting pummelo |
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| SONG Zhen;XU Xiao-feng;YANG Fang-yun;ZHOU Chang-yong.A p25/Hinf1 RFLP analysis of the Citrus tristeza virus isolates infecting pummelo[J].Plant Protection,2005,31(4):28-31. |
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| Authors: | SONG Zhen;XU Xiao-feng;YANG Fang-yun;ZHOU Chang-yong |
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| Institution: | Citrus Research Institute of Chinese Academy of Agricultural Science;Chongqing 400712;China |
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| Abstract: | Citrus Tristeza Virus(CTV) is one of the most economically important pathogen of citrus in many citrus producing areas of the world.Pummelo dwarf caused by CTV has become a serious problem in China recently.In order to discover their pathogenic characteristics,123 CTV isolates infecting pummelo were studied by p25/Hinf1 RFLP assay.Single p25/Hinf1 RFLP groups were detected in 82.9% of the 123 samples,and the dominant group was p25/Hinf1 RFLP group 6,which accounted for 79.7% of the total.There were 4 isolates belonging to p25/Hinf1 RFLP group 5,which could be mild CTV strains and should be further studied. |
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| Keywords: | phytovirology Citrus Tristeza Virus isolate RFLP assay |
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