A survey of antimicrobial-resistant Escherichia coli
prevalence in wild mammals in Japan using antimicrobial-containing media |
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| Authors: | Tetsuo ASAI Masaru USUI Michiyo SUGIYAMA Masako ANDOH |
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| Affiliation: | 1)The United Graduate School of Veterinary Medicine, Gifu University, Gifu, Japan;2)School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan;3)Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan |
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| Abstract: | The emergence and spread of antimicrobial-resistant bacteria and resistance genes pose serious human and animal health concerns. Therefore, to control antimicrobial-resistant bacteria in the environment, the status of antimicrobial resistance of Escherichia coli in a variety of wild mammals and their prevalence were examined using antimicrobial-containing media. In total, 750 isolates were obtained from 274/366 (74.9%) wild mammals, and antimicrobial-resistant E. coli was detected in 37/750 isolates (4.9%) from 7 animal species (26/366 [7.1%] individuals). Using antimicrobial-containing media, 14 cefotaxime (CTX)- and 35 nalidixic acid-resistant isolates were obtained from 5 (1.4%) and 17 (4.6%) individuals, respectively. CTX-resistant isolates carried blaCTX-M-27, blaCTX-M-55, blaCTX-M-1, and blaCMY-2, with multiple resistance genes. Fluoroquinolone-resistant isolates had multiple mutations in the quinolone-resistance determining regions of gyrA and parC or qnrB19. Most resistant isolates exhibited resistance to multiple antimicrobials. The prevalence of antimicrobial-resistant bacteria observed in wild mammals was low; however, it is essential to elucidate the causative factors related to the low prevalence and transmission route of antimicrobial-resistant bacteria/resistance genes released from human activities to wild animals and prevent an increase in their frequency. |
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| Keywords: | antimicrobial resistance Escherichia coli human activity medium wild mammal |
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