热应激对小鼠相关神经行为学影响

本研究旨在观察34℃条件下不同时长的热刺激对小鼠神经行为学影响及在此条件下小鼠血液生理指标的具体变化。选择40只8周龄小鼠，平均分为4组，每组10只。分组后将小鼠分别置于34℃环境下进行时长0、2、4、6 h的热刺激。刺激结束后立刻对小鼠进行旷场和高架十字迷宫试验，观察并记录小鼠5 min内运动情况，并采取小鼠血液样本对其血液生理指标进行检测。结果显示：与对照组相比，小鼠热刺激2 h组探索行为显著增加，而热刺激4 h组及热刺激6 h组小鼠焦虑样行为显著增加，探索行为减少；小鼠热刺激2 h组肾上腺素（EPI）、皮质醇（CORT）水平都呈时间依赖性增加，并且具有显著性；热刺激2、4、6 h组去甲肾上腺素（NE）水平均极显著增加，并在热刺激4 h时达到峰值，6 h时略有回落趋势；小鼠热刺激4 h组促肾上腺皮质激素释放激素（CRH）水平极显著增加，6 h时略有回落趋势。综上表明，34℃热刺激2 h时对小鼠自发运动和探索行为有促进作用，小鼠自发运动和探索行为随着刺激时长的增加，被显著抑制，焦虑样行为显著增加，表现出时间依赖性，并提示小鼠热刺激后情绪和行为异常可能由中枢神经系统进行调控。

Effect of Heat Stress on Related Neurobehavior in Mice

The purpose of this study is to observe the effects of different durations of thermal stimulation on the neurobehavior of mice at 34℃ and the specific changes in the blood physiological indicators of mice under this condition. Eight weeks old mice were selected and divide them into 4 groups with 10 mice in each group. After grouping, the mice were placed in a 34℃ environment for thermal stimulation for 0, 2, 4 and 6 hours. Immediately after the stimulation, the mice were subjected to open-field and elevated plus maze tests to observe and record the movement of the mice within 5 minutes, and take blood samples from the mice to detect their blood physiological indicators. Results were as follows:Compared with the control group, the exploratory behavior of the mice in the 2 h heat stimulation group was significantly increased, while the anxiety-like behavior of the mice in the heat stimulation 4 h group and the heat stimulation 6 h group was significantly increased, and the exploratory behavior was reduced; the levels of EPI and CORT of the mice in heat stimulation 2 h group increased in a time-dependent manner and the difference were significant; the levels of norepinephrine (NE) in the 2 h, 4 h, and 6 h heat stimulation groups increased extremely significantly, and it reached a peak at 4 h of heat stimulation and slightly decreased at 6 h; the level of corticotropin-releasing hormone (CRH) in mice in the heat-stimulated 4 h group increased significantly, and decreased slightly at 6 h. The results suggest that heat stimulation at 34℃ for 2 hours promotes spontaneous movement and exploratory behavior in mice. As the stimulation duration increases, the spontaneous movement and exploratory behavior of mice are significantly inhibited, and anxiety-like behaviors increase significantly, showing a time dependence, and suggest that the emotional and behavioral abnormalities of mice after thermal stimulation may be regulated by the central nervous system.